Clofentezine is an acaricide witch prevents fetus growing by contacting and ovicide.
This pesticide is so effective on excreting of seed of apple and pear red acary especially European red acary.
According to the tetrazine structure of colfentezine, there are many methods for syntheses of clofentezine that some methods was chosen for doing experiments.
The four step methods for preparation of clofentezine was used with 2-cholorobenzoyl chloride as a starting material that treated with hydrazine and produced N, N'-bis(2-chloro benzoyl) hydrazine.
The taken production chlorinated with PCl5 and FeCl3 as a catalyst to produce bis (a-2-dichlorobenzyliden) hydrazine, then treated with hydrazine hydrate and produced 1,2-dihydro- tetrazine ring.
The 1, 2-dihydro-tetrazine oxided to aromatic tetrazine ring in the presence of mixture of NaNO2/AcOH and produced clofentezine (yield=84%)
The other method is preparation of 1, 2-dihydro-tetrazine compound by refluxing of 2-chlorobenzonitryl and hydrazine hydrate, so clofentezine is produced with oxidation of 1, 2-dihydro- tetrazine ring (yield=27%).