Antibacterial effects of the extracts of myrtus on some gram positive bacteria (staph, aureus, Bacillusspp, Corinebacterium pyogenes) and some gram negatives bacteria (….) have been studied. The extract of the plant effectively inhibited the growth, of the above mentioned gram positive bacteria, but ineffective on gram negative.
In order to isolation and purification of the antibacterial materials from inactive materials of the plant, column chromatography and decantor crystallizing methods have been used. In view of extracting in decantor crystallizing method, solvents petroleum ether, ether, ethyl acetate,
ethanol and chloroform have been used and compared.
With the test, it has been determined that the amount of the crystals extracted with chloroform is much more than the other three solvents.
For isolation and purification of the extract column chromatography also have been used. By examining solvents. Silicagel with 0.2- 0.5mm mesh (pore size) and benzene and chloroform as the solvents have preferred.
In comparing the two methods of isolation and purification of the extracts it has also been determined that the method of crystallizing in the funnel of decantor is more suitable than column chromatography.
The methods infrared spectroscopy, nuclear magnetic resonance, mass spectroscopy for identification of extracted materials also have been used. The molecular structure is suggested for the active ingredient of the extract.