Introduction: This study was carried out to investigate if technology of in vitro embryo production can be used for protection and development of milk productivity potential of the superior cattle approached to ablation.
Material & Method: By cooperation of industrial husbandry complexes in Isfahan province, the ovaries of cattle selected for ablation were collected immediately after slaughter. Bovine immature oocytes were then used for in vitro maturation/fertilization and the presumptive zygote were then cultured for up to 8 days in Vero-TCM culture medium before being transferred into previously synchronized heifers of low genetic merits.
Results: From 21 superior cattle culled, the most causes of culling were related to reproductive problems (33.3%), lowest milk production related to systemic diseases (7824), most average of offspring production expected and number of offspring production were 9.2 and 7.4 in old cows, respectively and the most average of offspring not protection were 3 in reproductive problems cows. The most average oocytes collection were related to old cows and rate of cleavage was significant in each 4 groups and systemic diseases group (34.2%) was significantly lower than reproductive problems (69.6%) and mastitis (60.6%) groups in 5-8 cells stage. Systemic diseases (36.6) were significantly lower than reproductive problems (73.9) groups in 8-16 cells. Rate of morula was 71.8% in reproductive problems group and rate of blastocysts old cows (34.2%) was significantly higher than other groups. Two established pregnancies (25%) and one viable off springs (50%) were resulted from 8 blastocysts transferred.
Conclusion: The advances of assisted reproductive technology (ART) can effectively protect and multiply the superior genes of lactation and reproduction within the dairy farms.