Paper Information

Title: 

EFFECT OF NITRIC OXIDE ON THE INTEGRITY OF FEMALE REPRODUCTIVE SYSTEM IN THE OVARECTOMIZED RATS

Type: POSTER
Author(s): HABASHI H.*,NAJAFPOUR A.,SADEGHI HASHJIN G.
 
 *FACULTIES OF VETERINARY MEDICINE, ISLAMIC AZAD UNIVERSITY OF URMIA
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: In the male reproductive tract, nitric oxide (NO) is an important factor involved in erectile function. Nevertheless, the importance of NO in the integrity of the female reproductive system is not known. This study was designed to investigate the possible effect of NO on the integrity of uterus after ovary removal in rats.
Methods: Twenty five female adult Wistar rats weighing 200-250 g were divided into 5 groups as follows: negative control (preliminary operation without ovariectomy), positive control (ovariectomy + saline treatment, NTG (ovariectomy + nitroglycerine 1 mg/kg), L-Arg (ovariectomy + L-arginine 10 mg/kg), L-NAME (ovariectomy + L-NAME 0.75 mg/kg). Treatments were performed twice daily for 10 days s.c. On the day 11, rats were anesthetized and blood samples were collected for CBC. Thereafter, animals were euthanized and the reproductive tract (vagina, cervix and uterus) were removed for wet weight determination. Later, the tissues were dried (80؛C, 24 hours) for dry weight determination.
Results: The prominent alteration in the CBC was increased number of platelets in all ovariectomized animals, not affected by later treatments. A partially decreased monocytes only in the L-Arg and NTG treated rats could be induced by NO. Wet and dry weights of the genital tract were significantly (<50%) decreased in the NTG-treated rats compared with positive controls (P<0.05). In L-Arg treated animals, weight of the reproductive tract was slightly decreased.
Conclusion: The novel finding is a degenerative effect of NO in female genitalia, particularly in high concentrations. More research work should be performed to investigate if it could occur under clinical conditions, i.e. in the ovariectomized women receiving nitro-vasodilators.

 
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