Paper Information

Title:  DEVELOPMENT OF A FAST METHOD TO DETERMINE CALCIUM CHANNEL BLOCKERS EFFECT ON OXYGEN-GLUCOSE DEPRIVATION IN FETAL CD1 MICE NEURONAL PRIMARY CELL CULTURE
Type: POSTER
Author(s): TAVAKOLIFAR B.*,RAHBAR ROUSHANDEL N.,RAHIMI MOGHADAM P.,MAHMOUDIAN M.
 
 *DEPARTMENT OF PHARMACOLOGY, IRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Calcium overload is involved in neuronal injury occurring in cerebral hypoxia or ischemia. To clarify further the role and the origin of calcium in cerebral ischemia, we developed an in-vitro model of glucose-oxygen deprivation to see effects of various dihydropirydines such as nimodipine.
Methods: Mice fetal cortical primary cell cultures were prepared from 15-day embryonic animal. Following an aseptic dissection of cerebral cortices, dissociated cells were placed on poly-L-lysine-coated 96-well plates .All cultures were grown for 8-12 days. Cultures media were replaced by glucose-free media and were placed in an oxygen-free jar for 30 minutes (OGD conditioning). Various concentrations of nimodipine were added to the incubation media and 24 h before oxygen glucose deprivation. Cellular viability was assessed by MTT assay.
Results: In our model, glucose oxygen deprivation decreased the number of viable cells significantly. Nimodipine increased cell viability in neuronal cells exposed to oxygen-glucose deprivation.
Conclusion: The mice fetal neuronal cell culture in 96-well plates exposed to glucose-oxygen deprivation could be used as a fast method to determine the effect of calcium channel blockers.

 
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