Paper Information

Title: 

ROLE OF CONTEXTUAL CUES IN ESTABLISHING PLACE CONDITIONING

Type: POSTER
Author(s): AZHDARI ZARMEHRI H.*,CAGNIARD B.,MURPHY N.P.
 
 *RIKEN BRAIN SCIENCE INSTITUTE, JAPAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Memories for drug-paired cues are responsible for much of the relapse behavior seen in addicts. Conditioned place preference (CPP) is a well-established model for studying the rewarding effects of drug-associated cues.
Methods: We studied drug-paired cues in 5 new apparatuses with
different multimodal contextual cues: 1-Tactile (smooth and grid floors) and visual cues (black and white check and cow patterns) 2- As #1 but also with olfactory cues (almond and lemon odors). 3- As #2, but with neutral tactile cues. 4- As #2, but with punched steel and gird floor tactile cues. 5- As #2, but with textured rubber and grid floor tactile cues. We evaluated sensitization, CPP and conditioned place aversion (CPA) by repeated administration of methamphetamine (rewarding drug, 2mg/kg) and naloxone (aversive drug, 10mg/kg). Male C57BL6 mice were divided into: vehicle, methamphetamine- and naloxone-conditioned. The experimental process consisted of: 1- A free-choice pre-test session (20 min), 2- Alternating conditioning sessions over six days (40 min each, three vehicle, three drug) and 3- A free-choice test session (20 min). Results: Naïve mice spent approximately 50% time in each compartment during the pretest. Thus, the apparatuses can be considered unbiased. In all studies, methamphetamine induced behavioral sensitization (p<0.001). Methamphetamine pairing with the grid floor only induced a CPP in experiment 1. Methamphetamine induced a CPP in both compartments (p<0.05) after changing the tactile cues. Mice showed a CPA to the naloxone-paired floor regardless of the cue it was paired with (p<0.001).
Conclusion: Tactile cues are important for establishing methamphetamine induced CPP possibly due to differences in sensation and perception to the tactile signal when in a methamphetamine-drugged state.

 
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