Deoxynivalenol (DON) is one of the most important mycotoxins which are produced by Fusarium fungi. The occurrence of this mycotoxin has been reported in some cereal products around the world. There are also some mycotoxicosis cases due to use of contaminated bread flour with this mycotoxin. Since the flour is highly important in bakery products, therefore this study carried out in order to measure DON in two different flours (those mostly used for bread). One kilogram samples of flours (debranned & Setareh flour) were taken from some flour factories selected randomly in Tehran province. The samples were transferred to laboratory and divided into 100 and subsequently into 10 gram of sub samples. Then the samples were extracted by immunoaffinity columns and analyzed with HPLC instrument which was equipped with UV detector and C18 column. Mobile phase was Acetonitrile – Water in the ratio 10-90 (V/V).
DON was detected in all twenty two samples of two kinds of flours (Setareh and debranned). The mean contaminations of DON in these two flours were more than the limit value (0.5 – 4ppm) which is set by some countries. Statistical analysis of data showed that there was no significance difference between these two kinds of flours.