Paper Information

Title: 

THE EFFECTS OF MORPHINE AND NALOXON ON SPONTANEUSE SEIZURE ACTIVITY IN HIPPOCAMPAL SLICES

Type: POSTER
Author(s): ESMAEILI M.H.*,HAGH DOUST H.,GHEYBI N.A.
 
 *DEP OF PHYSIOLOGY, QAZVINE MEDICAL UNIVERSITY, QAZVINE, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Background: opioid peptides have been shown to be involved in the mechanisms by which seizure activity spontaneously terminates. Opiates have complex effects on seizure activity .Both anti-and proconvulsive actions have been reported depending on the exact experimental conditions.
Objective: To determine the effects of different dose of morphine and Naloxon on spontaneous seizure activity in epileptogenic hippocampal slices.
Methods: For this, Hippocampal slices (~400 µm) were prepared from young Wistar rats (P10- 25). Seizure activity was induced by continuous per fusing of the slices with low -Mg2+ ACSF. Extra cellular recordings were performed in the hippocampal CA1 layer. Seizure activity was quantified by measuring the amplitude and duration of the ictal events as well as their number before and after the application of the study drugs.
Findings: Our results show that Low doses of morphine (10 µM) suppressed seizure activity, whereas high doses of morphine (30 & 100 µM) potentiated seizure activity in a dose dependent manner. This effect was completely reversed by the addition of Naloxon (10μM). Differential effects of morphine on seizures, suggests that morphine in different concentrations acts on different receptor subtypes or by different mechanisms. It is likely that activation of opioid receptors by morphine lead to suppression of GABAergic synaptic transmission thereby disinhibiting pyramidal neurons, resulting in the enhancement of the low-Mg2+ ACSF induced seizures. Conclusion: our result together with the previous reports indicates that the effects of morphine-like drugs on seizure are site -method- dose - age - sex dependent.

 
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