Paper Information

Title: 

METHANOL EXTRACT OF SAFFRON AMELIORATE EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS IN MICE

Type: POSTER
Author(s): ESLAMBOLCHI SH.*,ROSTAMI PARVIN,SANATI M.H.,HAGHBIN K.A.D.
 
 *BIOLOGY DEPT., BASIC SCIENCE FACULTY, TARBIAT MOALLEM UNIV., TEHRAN, I.R., IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Multiple sclerosis is an inflammatory demyelinating disease of central nervous system and experimental autoimmune encephalomyelitis (EAE) is commonly used as an animal model for it. There are some evidences in folk medicine and recent pharmachological experiments for antinociceptive and anti-inflammatory effects of saffron (crocus sativus L.).
Methods: C57BL/6 mice were inoculated by the peptide myelin oligodendrocyte glycoprotein (MOG) in complete freund’s adjuvant subcutaneously. Pertusis toxin was injected intraperitoneally in day inoculation. The animals were observed daily for signs of EAE. Saffron stigma methanol extracts (25, 50, 100, 500, 1000 mg/kg) and aqueous extracts (50, 100, 200, 400, 800 mg/kg) and dexamethasone (0.5 and 1 mg/kg) were administered intraperitoneally to experimental groups respectively. Control group was given PBS solution (100
ml/mouse). One group considered as sham was immunized was without any other treatment. Treatments continued for 15 successive days.
Results: Results have shown that 100mg/kg, 500mg/kg and 1000 mg/kg of methanol extracts suppressed mean clinical scores, mean maximum severity, and disease index significantly in compare with less doses, PBS-treated and sham groups (P≤0.001). A similar suppression was observed with dexamethasone. Administration of aqueous extracts did not show any suppressive effects on clinical symptoms (P≤0.001).
Conclusion: Since crocus sativus L. is contained many carotenoids and flavonoids, the present results suggest that saffron methanol extract play a protective role for central nervous system against relapses in EAE in mice.

 
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