Paper Information

Title: 

T-2 TOXIN HEPATOTOXICITY IN THE LIVER PERFUSED RAT MODEL

Type: SPEECH
Author(s): DARAIE B.*,RASEKH H.R.,GHAZI KHANSARI M.
 
 *DEPARTMENT OF PHARMACOLOGY, TOXICOLOGY SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, AND DEPARTMENT OF PHARMACOLOGY, SCHOOL OF MEDICINE, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF TOXICOLOGY
Type of Seminar:  CONGRESS
Sponsor:  SOCIETY OF TOXICOLOGY
Date:  2004Volume 8
 
 
Abstract: 

T-2 toxin, a trichothecene mycotoxin, is considered one of the most toxic compounds that are produced by molds, particularly the Fusarium species. Contamination of cereals such as barley, wheat, rice and maize with Fusarium mycotoxins has been reported worldwide. The isolated perfusion rat liver (IPRL) was chosen because it permits studies of liver function in an in situ system that resembles normal physiology.
In this study، male Sprague dawley rat was used. It is shown that T-2 toxin causes alterations in biochemical parameters in IPRL. Results have shown that T-2 toxin increased aminotransferase activity, lipid peroxidation, and dose-dependently increased carboxylestrase activity. Meanwhile, results have showed a significant increase in both AST and ALT activities in all concentrations compared with control (p<0.01, p<0.05 respectively). Different concentrations of T-2 toxin (4, 9, 21, 43ρmol/L) caused a significant decrease in GSH (glutathione) level in liver homogenates as compared to control (p<0.01). It was also shown that addition of the toxin to perfusion buffer resulted in a significance decrease (p<0.05) in GSH concentration in billiary excretion in doses of 21 and 43ρmol/L as compared with their respective control. It is possible that T-2 toxin reduces the level of GSH when is required for the elimination of peroxide radicals.
The TBARs values increased in perfused liver 2hr after administration of T-2 toxin reaching values approximately six (21ρmol/L) and seven (9, 43ρmol/L) times higher than control. This study showed that T-2 toxin causes an increase in lipid peroxidation while causing a decrease in GSH in the IPRL. GSH and lipid peroxidation are both sensitive indicators of oxidative stress. The TBARs values were maximal 2h after the T-2 challenge. The histopathologic examination of perfused rat liver have shown that T-2 toxin dose dependently caused an increase in dilatation, swollen hepatocytes, inflammation and necrosis in rat liver.

 
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