Paper Information

Title: 

ANTISPASMODIC EFFECT OF PIPER NIGRUM FRUIT AQUEOUS EXTRACT ON RAT ILEUM

Type: POSTER
Author(s): GHARIB NASERI MOHAMMAD KAZEM*,YAHYAVI H.
 
 *DEPT. OF PHYSIOLOGY, AHWAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES, AHWAZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Piper nigrum (Black pepper) from Piperaceae is main constituent of food spice and piperine as its main component increases bile and small intestine secretions, but reduces the gastric emptying and increases intestine transit time in rat and mouse. In Iranian traditional medicine, black pepper is used to relief the menorrhalgia in women. This study was performed to investigate the effect of black pepper fruit aqueous extract on rat ileum contractions caused by spasmogens and the mechanism(s) its action.
Methods: Aqueous extract of black pepper was prepared and its effect on precontracted ileum was examined.
Results: Cumulative concentrations of black pepper aqueous extract (0.0625-1 mg/ml) reduced the ileal contractions induced by KCl (60 mM) and carbachol (10
mM) dose dependently (P<0.0001). The spasmolytic effect of extract on the KCl-induced contractions were unaffected by ileum incubation (30 min, 1 µM) with propranolol and even potentiated by incubation with naloxone (30 min, 1 µM) and L-NAME (20 min, 100 µM). Potassium channels blockers, glibenclamide and tetraethylammonium (5 min, 10 µM and 1mM respectively) did not reduce the extract antispasmodic effects in carbachol-induced ileal contractions. In calcium free Tyrode solution with high potassium (60mM), extract (0.0625-1mg/ml) reduced the contractions induced by cumulative concentrations of calcium chloride (0.225-2.7mM) dose dependently (P<0.01- P<0.0001).
Conclusion: Our results suggest that the spasmolytic effect of black pepper aqueous extract on rat ileum was mediated via voltage dependent calcium channels. However, the
b-adrenoceptors, opioid receptors, nitric oxide generation, potassium channels activation were not involved.

 
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