Paper Information

Title: 

ACCLIMATION OF DUNALIELLA SALINA (POTENT SOURCE OF β-CAROTENE) TO EXTREME CONDITION LOW IRRADIANCE AND CARBON DIOXIDE

Type: POSTER
Author(s): HOSSEINI K.Z.*,SATEI Y.,SHOKRAVI SH.,SAFAEI M.,AMIR LATIFEI F.,HOSSEINI E.
 
 *DEPARTMENT OF BIOLOGY, ISLAMIC AZAD UNIVERSITY, GORGAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: b-carotene is the presersor of vitamin A.This compound may be considered as a potent anticancer metabolit. The effect of b-carotene to decrease the frequency of cancers has been emphasized in a great amount of immunological and epidemiological reserches. Considering that more than 10% of the dry weight of Dunaliella strains may be composed by b-carotene, studying of viability and acclimation of these strains to environmental condition especially extreme one, seem basic from both pore and applied point of views. In this research acclimation to very low light intensity (beloww 1000 lux) and limited, dissolved inorganic carbon, have been studied in D.salina. A potent strain of microalga especially from industrial biotechnological aspects.
Method: After collection and preliminary identification, the strain was incubated under 30C, very low continuous irradiance (980 lux), and with usuall aeration condition. Salt treatments 0%, 7.5%, 15%, 30% and 50% were made using NaCl in usuall Johnson media in the batch form. Growth was monitored by turbidity using OD750. Pigment composition (carotene and xanthphylls) and concentration were measured after acetone extraction.
Results: Data showed that the highest rate of growth may be seen at 7.5% salt concentration. The highest rate at carotene and xanthophylls production may be relate to 30% salt concentration. The strain could be keep viability and growth at this extreme condition.
Results: Itseem interesting that the strain can be acclimate to such as extreme condition (very low irradiance and limited carbon dioxide). This may be considered as an important find especially from economic point of view. It seems that CO2- Concentration mechanism must be completely active at this condition, as in addition the strain may be adapt to hard condition using flexible pigment concentration ability.

 
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