Paper Information

Title: 

EVALUATINGTHE ANESTHETIC EFFECT OF CLOVE ESSENCE IN MICE

Type: POSTER
Author(s): DASHTI M.H.,MORSHEDI A.,NOUR ALDINI S.J.,SALAMI A.S.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Anesthesia is an important step in many experimental animal studies.. The drugs currently used as anesthetics are usually expensive and also are not safe for all species. Previous studies have reported the effect of Clove extract as a local sedative in toothache, and as an anesthetic drug in aquatics such as Trouts. In the present study we conducted to evaluate the anesthetic effect of Clove essence in mice.
Methods: In this study Syrian mice weighting 25-30 g were used. Different doses of Clove essence were injected intrapritonealy. Each dose was administered for 10 animals. The anesthetic effect was evaluated immediately after the injection of Clove essence according to the expression of motion impairment or Imbalance, akinesia, tail pinch unresponsiveness (as an index of complete anesthesia) and recovery to normal motion.
Results: Our findings showed that Clove essence in doses equal or less than 1 ml/kg did not produce complete anesthesia, but the administration of doses equal to or greater than 1.5 ml/kg in a dose dependant manner revealed a period of complete anesthesia. The dose - response curve for different anesthetizing doses was plotted and 1.8 ml/kg was determined as ED50. Mortality was observed by administrating 2.5 ml/kg Clove essence and by considering the mortality rate induced by different doses from initial to full lethal dose, LD50 for Clove essence was 2.8 ml/kg.
Conclusion: although Clove essence induces anesthesia in mice since its ED50 and LD50 are very close to each other this herb med is not a very safe choice for anesthesia induction in mice.

 
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