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Paper Information

Title: 

ANTISPASMODIC ACTIVITY OF APIUM GRAVEOLENS LEAF EXTRACT ON RAT ILEUM

Type: POSTER
Author(s): GHARIB NASERI MOHAMMAD KAZEM,PILEHVARAN A.A.,SHAHMANSOURI N.*
 
 *DEPT. OF BIOLOGY, ISFAHAN PAYAME NOOR UNIVERSITY, ISFAHAN, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Celery (Apium graveolens) from Aliaceae contains apigenin and isoquercetin. This herb has anti-inflammatory activity and in traditional medicine is used as diuretic and antispasmodic remedy. The aim of present study was to investigate the antispasmodic effect of Apium graveolens leaf extract on rat ileum contractility.
Methods: Apium graveolens leaf hydroalcoholic extract (ALHE) was prepared by mixing celery leaf powder with 70% alcohol for 72 h at room temperature using maceration method. Solvent was evaporated and extract powder was obtained. A terminal portion of male rat ileum was dissected and its contractions were recorded isotonically in an organ bath containing Tyrode solution (10 ml, pH 7.4, 37 ؛C) under 1 g tension.
Results: cumulative concentrations of ALHE (0.5, 1, 2, 4 and 8 mg/ml) reduced the ileum contraction induced by KCl (60mM) and by carbachol (10μM) dose-dependently (P<0.0001). The antispasmodic effect of ALHE was not reduced by tissue incubation with propranolol (30 min, 1μM), naloxone (30 min, 1μM), L-NAME (20 min, 100μM). In Ca2+-free with high K+ (60mM) Tyrode solution, ALHE (1, 2 and 4mg/ml) reduced the ileum contractions induced by CaCl2 (0.5, 1, 2, 4 and 8mM) dose-dependently (P<0.01-P<0.0001).
Conclusion: Apium graveolens hydroalcoholic leaf extract inhibits the ileum contractions without involving β-adrenoceptors, opioid receptors, NO synthesis. It seems that the calcium channels are involved in the spasmolytic effect of the extract.

 
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