Paper Information

Title: 

RELAXETORY EFFECT OF VITIS VINIFERA LEAF POLYPHENOL ON RAT AORTA

Type: POSTER
Author(s): HEYDARI A.*,GHARIB NASERI MOHAMMAD KAZEM,RAMEZANI Z.
 
 *DEPT. OF PHYSIOLOGY AND DEPT. OF CHEMISTRY, AHWAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: The benefit and protective effects of polyphenols such as flavonoid, is proven by epidemiologic researches and some mechanisms are suggested for their bioactive effects. Our previous studies showed that Vitis vinifera hydroalcoholic leaf extract relaxed the phenylephrine-induced contraction in rat thoracic aorta. This effect was involved NO-cGMP system. However, the effective compounds were not investigated.
Method: Extract was prepared by suxhlet method using 70% alcohol. Dry extract dissolve in 70% methanol and fats were separated with petroleum ether in decantor. Nonpolar compounds were separated with chloroform then poly phenols were separated from remaining extract with ethylacetate. Thoracic aorta of Wistar male rat (160- 210 g) was mounted in organ bath (Krebs- Henseleit solution, 37
˚C, pH 7.4) and aortic contractions were recorded isometrically under 1 g resting tension. The aorta endothelium was accepted intact if acetylcholine (1μM) induced 70% relaxation on phenylephrine (1μM)-induced contraction.
Results: The results showed that Vitis vinifera leaf polyphenols (0.2, 0.4 and 0.8 mg/ml) reduces phenylephrine- induced contractions (P<0.001) and dose dependently. The vasorelaxant effect of Vitis vinifera leaf poly phenols was reduced by L-NAME (100μM, 30 min, P<0.001) and methylene blue (10μM, 30 min, P<0.05).

Conclusion: The results suggest that, Vitis vinifera leaf polyphenols induce relaxation in rat aorta dose dependently and No-cGMP system was involved.

 
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