Paper Information

Title: 

MODIFIED FORMULATION OF MEDIUM CULTURE FOR HELICOBACTER PYLORI & ANTIMICROBIAL EFFECTIVITY OF NEPETA UCRAINICA L. SUBSP. KOPETDAGHENSIS AGAINST HELICOBACTER PYLORI

Type: POSTER
Author(s): KHAJEH KARAM ALDINI M.*,RAMEZANI MOHAMMAD,GHOLOUBI I.,HOSSEINABADI T.
 
 *
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

In this study, the effect of addition of L-cystein to culture medium on the growth of Helicobacter pylori and the inhibitory effect of total extract of Nepeta ucrainica L. subsp. Kopetdaghensis on this bacterium were evaluated. The dried and powered plant was extracted with methanol in suxhlet apparatus. Different concentrations of extract (25, 50, 100 and 200 mg/ml) in methanol were prepared and tested against clinical isolates of H. pylori using agar diffusion method. Methronidazole (30 mg/ml) was used as positive control. All plates were kept in jars under microaerophillic conditions at 37°C for 5-7 days. The zone of inhibition was measured after one week. The effect of cysteine on growth of H. pylori was studied by adding 1 mg/ml of cysteine to Colombia agar culture medium. The number and the size of colonies were compared in two cysteine-free and cysteine containing media. The zone of inhibition of extract at 25, 50, 100 and 200 mg/ml and metronidazole at 30 mg/ml on 15 clinical isolates were 5.6, 8.8, 12.3, 17.0 and 17.2 mm respectively, indicating a significant activity compared to metronidazole. The MIC determined by agar dilution method was 6.6 mg/ml for all isolates. The addition of cysteine showed a much better growth in all cases and the extract of plant in concentration more than 100 mg/ml had considerable inhibitory effect on H. pylori.

 
Keyword(s): 
 
Yearly Visit 29   tarjomyar
 
Latest on Blog
Enter SID Blog