Paper Information

Title: 

EFFECTS OF THYMOQUINONE ON EPILEPTIFORM ACTIVITY INDUCED BY MAGNESIUM FREE MEDIUM IN THE CA1 REGION OF RAT HIPPOCAMPAL SLICES

Type: POSTER
Author(s): PARVARDEH S.*
 
 *DEPT. OF PHARMACOLOGY, SCHOOL OF MEDICINE, AHVAZ UNIVERSITY OF MEDICAL SCIENCES.
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: It has been recently shown that thymoquinone, the major constituent of Nigella sativa seeds, suppresses epileptic seizures in mice and rats. In the present study, electrophysiological experiments were carried out in order to further investigating of thymoquinone effects on epileptiform activity induced by magnesium (Mg2+)-free medium in rat hippocampal slices.
Methods: After decapitation of rats under Isoflurane anesthesia, the brain was removed and the hippocampus was dissected out. Then, 500-M transverse hippocampal slices were prepared using a vibratome. In order to elicit epileptiform activity in the hippocampus, the slices were perfused with Mg2+-free artificial cerebrospinal fluid (ACSF). Then evoked epileptiform activity and spontaneous interictal spikes were recorded extracellularly from the CA1 pyramidal neurons of hippocampus. Finally, the effects of thymoquinone (10-1000
mM) on the amplitude or duration of epileptiform potentials were evaluated.
Results: The results showed that thymoquinone (100
mM) decreased the amplitude of secondary spikes in evoked epileptiform discharges, as well as the amplitude and frequency of spontaneous interictal spikes.
Conclusion: These Findings indicated the antiepileptiform activity of thymoquinone in vitro, so that thymoquinone suppressed the both evoked and spontaneous epileptiform discharges induced by Mg2+-free ACSF in the rat hippocampal slices.

 
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