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Paper Information

Title: 

17 β-ESTRADIOL INHIBITS CALCIUM-DEPENDENT AND INDEPENDENT CONTRACTION IN ISOLATED HUMAN SAPHENOUS VEINS

Type: POSTER
Author(s): AZARMI Y.*,BABAEI H.
 
 *FACULTY OF PHARMACY, TABRIZ UNIVERSITY OF MEDICAL SCIENCE
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction In: In previous studies demonstrated that 17b-estradiol (B-EST) inhibits calcium-dependent, but not calcium-independent, vascular contraction. The aim of this study was to evaluate the effects of B-EST on calcium-dependent and -independent contractions in human saphenous veins (HSV) rings
 Methods: HSV were obtained from patients undergoing coronary artery bypass grafting in Madani Heart Center of Tabriz. Rings of HSV were prepared; the endothelium was removed by mechanical ablation and equilibrated in Krebs (37oC; carbogen).The ability of B-EST to modulate Ca2+ entry was assessed by obtaining concentration-response curve to CaCl2 in the absence or presence of the B-EST in depolarizing medium. In other experiments intracellular Ca2+ was depleted in the presence of cyclopiazonic acid in Ca2+-free Krebs with repeated application of phenylephrine. Following depletion of intracellular Ca2+, PGF2α was administered, at the plateau of contraction B-EST or nifedipine was added. The role of PKC in relaxation effect of the B-EST was evaluated by contracting vein rings with phorbol dibutyrate (PDBu) in normal Krebs’ and Ca2+-free Krebs’ solution in a separate series of experiments. When a stable contraction was obtained B-EST or nifedipine was added for 40 mins.
Results: Pretreatment with B-EST concentration-dependently reduced vein contractions induced by calcium chloride in the Ca2+ free solution containing KCl. B-EST elicited relaxant effects on the PGF2α induced contractions in Ca2+ free solution in the presence of cyclopiazonic acid. Both B-EST and nifedipine produced significant relaxation in human saphenous vein rings contracted by direct activation of PKC by PDBu (10nM) in normal Krebs, but in Ca2+- free only B-EST could elicit relaxant effect on the PDBu (100nM)-induced contractions.
Conclusion: These results suggest that B-EST inhibits Calcium-dependent and –independent HSV contraction.

 
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