Paper Information

Title: 

RENAL DYSFUNCTIONS IN CONSCIOUS RAT WITH LITHIUM-INDUCED NEPHROGENIC DIABETES INSIPIDUS

Type: SPEECH
Author(s): HAGHANI M.,SHAHED MOUSAVI S.M.,KARIMI Z.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: The use of lithium, as the drug of choice for treating bipolar affective disorders, is frequently complicated by impaired renal water reabsorption resulting in development of nephrogenic diabetes insipidus (NDI). The aim of present study was to find out alterations in renal hemodynamic and excretory functions in conscious rats with lithium-induced NDI.
Methods: For NDI-induction, male Sprague-Dawely rats (225-265g, n=7) were given food containing 40 and 60 mmol LiCl/kg for 4-5 weeks, tap water and normal saline was available ad libitum. In the control group (n=6), rats (174-210g) were fed standard rat pellets for a period matched to the NDI group. However, three days before putting them into metabolism cages, low amount of LiCl (12 mmol/kg) was added to their diet to give measurable [Li]P of 0.1-0.2 mM. Each rat was individually placed in metabolism cage for collecting urine and measuring fluid intake during 6 h, and finally an arterial blood sample was taken.
Results: Comparison of data between NDI and control groups showed increases in fluid intake (8.8±0.42 vs 0.63±0.15 ml/h, (P<0.001) , absolute Na excretion (15.7±3.3 vs 4.8±1.4 micromol/min. kg(P<0.01) urine flow rate (562.0±34.5 vs 28.8±5.4 microl/min.kg, (P<0. 01) and free water clearance (302.0±40.7 vs -162.0±65.3 microl/min.kg, (P<0.001) but decreases in fractional distal water reabsorption (72.3±3.0 vs 95.3±2.5%, (P<0.001) and UOsm (139±15 vs 1730±246 mOsm/kg H2O, (P<0.001); while there were no differences in GFR as well as absolute and fractional proximal water reabsorption.
Conclusion: In conscious rats, lithium-induced NDI was associated with no alterations in glomerular filtration and proximal tubular reabsorption, but with reduced distal free water reabsorption, which was the cause of low osomolality polyuria with natriuresis.

 
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