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Paper Information

Title: 

EFFECT OF A POLYSACCHARIDE FROM A MEDICAL HERB, THE ROOTS OF BUPLEURUM FALCATUM L. ON INTERLEUKIN 6 PRODUCTION OF MICE B CELLS

Type: SPEECH
Author(s): MORSHEDI AHMAD,SAGHAEI P.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

The roots of Bupleurum falcatum L. have been widely used for the treatment of inflammatory disease in Japanese herbal medicine. Recently, the pharmacologically active polysaccharide, bupleuran 2 IIb and 2 IIc have been isolated from the hot water extract of the root of B. falcatum. Recently, it has demonstrated that bupleuran 2IIc also induced the proliferation and maturation of mice B cells. In the present study, the effect of bupleuran 2 IIc in IL - 6 production of murine B cells in order to know the mechanism of Ig secretion - enhancing activity of bupleuran 2 IIc was investigated.
Methods: Bupleurum 2 IIc extracted from pectic polysaccharide fraction was purchased from Pharmacia fine chemicals, Sweden. The Lymphocytes were isolated from 5 mice spleens, and the B cells were purified from spleen cell by magneting cell sorting system with using of anti - mouse CD 45R/B220 Mice Beads (100 Ab/108 spleen cells). Purified B cells at 2 106 cells / ml in RPMI medium culture solution were put in 24 well micrplate in four groups. The cells in each group were treated with or without bupleuran and anti-IL-6. The level of IgM and IL-6 in supernatant of B cell culture groups were assayed by ELISA .
Results: The total IgM amounts in a, b, c and d groups was obtained whit mean OD of 0.90/ 0.60 / 0.35 and 0.41 respectively. The amounts of IL - 6 in a and d groups, with as without bupleuran 2 IIc, were 1450 and 500 pg/ ml respectively.
Conclusion: The present study showed that secreted IL - 6 from B cells contributed to the enhancement of IgM production from bupleuran 2 IIc - treated B cells. On the other hand, because IgM production was reduced significantly (p<0.005) by anti - IL 6 Ab in B cells treated with bupleuran 2 IIc, suggesting IL - 6 involves in IgM secretion - enhancing activity of bupleuran 2 IIc.

 
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