Paper Information

Title: 

DIFFERENT EFFECTS OF INTRAPLANTAR AND SYSTEMIC ADMINISTRATIONS OF MORPHINE ON SPINAL G-PROTEIN GENE EXPRESSION LEVELS IN SPINAL RAT

Type: SPEECH
Author(s): ASKARI NAYEREH*,MAHBOUDI FEREYDOUN,HAERI ROUHANI ALI,YARI R.,KAZEMI B.,MAGHSOUDI NADER,AHMADIAN A.A.H.
 
 *
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Opiates are considered as the most powerful analgesic in clinic. All three types of opioid receptors have been found on peripheral terminals of sensory neurons. Changes in the resting levels of G-protein subtypes could have an effect on intracellular signaling pathways. Our previous results have shown that acute systemic administration of analgesic doses of morphine could change the G-protein genes expression levels. The current study was designed to investigate whether the systemic and local administration of analgesic doses of morphine differently affect on the level of G-protein subunits gene expression in rat spinal.
Methods: The gene expression of G-proteins was assessed by using real time PCR.
Results According to the present data, a significant elevation in the mRNA levels of Gi
a was observed in the group that received analgesic dose of morphine systemically while intraplantar treatment with analgesic dose of morphine had no effect on the Gia gene expression. Systemic and local Administration of analgesic doses of morphine in the presence of carrageenan induced different alterations in the levels of Gia mRNA. Systemic injection of analgesic dose of morphine before carrageenan administration decreased the mRNA levels of Gs compared to the carrageenan group but enhanced in compared to control group. While our results detected the significant decrease of Gs subunit mRNA levels following the local administration of morphine before carrageenan injection compared to the both control and carrageenan groups.
Conclusion: Changes in gene expression following exposure to systemic administration of opiates are likely responsible for the alterations in responsiveness of opioid sensitive neurons.

 
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