Paper Information

Title: 

THE CONSTRUCTION OF A NOVEL PROINSULIN WITH MINI C-PEPTIDE FOR DEVELOPMENT OF RECOMBINANT INSULIN PRODUCTION

Type: PAPER
Author(s): ABACHI M.*,OMIDI MARYAM,ALI AHMADI ATOUSA,MORADI ALI,TAHERIEH A.,SADEGHCHEH T.,JEBELI M.R.,AZAMI MEHRNAZ,SARDARI SOROUSH,MAHBOUDI FEREYDOUN*
 
 *BIOTECHNOLOGY RESEARCH CENTER, PASTEUR INSTITUTE, TEHRAN, IRAN
 
Name of Seminar: NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN
Type of Seminar:  CONGRESS
Sponsor:  IRANIAN BIOTECHNOLOGY ASSOCIATION
Date:  2005Volume 4
 
 
Abstract: 

To increase the folding efficiency of the proinsulin and the production yield of insulin, we have displaced C- peptide of proinsulin with the sequence EAEDLQ. The instability index of mini- proinsulin and fusion part is computed to be 26.38. This classifies the protein as stable. The mini-proinsulin was fused at the N-terminus to thioredoxin fusion partner containing a His6 tag for affinity purification. The gene for the fusion protein was inserted downstream of the T7 promoter of the expression plasmid pET-32a. The observed results from native PAGE, ELISA and HPLC show improvement in recombinant insulin production.

 
Keyword(s): MINI PROINSULIN, RECOMBINANT, ORIGAMI B
 
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