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Paper Information

Title: 

STUDY OF EFFECT OF PRALIDOXIME ON HUMAN SERUM BUTYRYLCHOLINESTERASE ACTIVITY IN THE ABSENCE AND PRESENCE OF PARATHION AND PARAOXON

Type: SPEECH
Author(s): JAFARI M.,POURHEIDARI GH.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF TOXICOLOGY
Type of Seminar:  CONGRESS
Sponsor:  SOCIETY OF TOXICOLOGY
Date:  2004Volume 8
 
 
Abstract: 

Organophosphorus insecticides (parathion and its major metabolites, paraoxon) are widely used in agriculture. They are potentially toxic to man because of their capacity to inhibit cholinesterases. Human serum Butyrylcholinesterase (BuChE) is important because in hydrolyzes a wide range of toxic compounds. Pralidoxime is one of the antidotes that used for organophosphate poisonings. In this study, human serum was separated from the blood of healthy volunteers, exposed to various concentrations of parathion (1-10µM) and paraoxon (0.03-0.3 µM) in combination with pralidoxime (100-300µM) and incubated for 10 min. After incubation, BuChE activity and percentage inhibition of the enzyme activity was measured by biochemical methods. The results show that in the presence of pralidoxime alone, raising pralidoxime concentration increased BuChE activity. Also, in the presence of pralidoxime, the effect of parathion and paraoxon on BuChE inhibition was recovered. However, at 300µM concentration of pralidoxime, not only recovered BuChE inhibition but also BuChE activity was higher than the control.

 
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