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Paper Information

Title: 

SEQUENCE ANALYSIS OF RESURGENT PLASMODIUM VIVAX POPULATIONS IN NORTHERN PART OF IRAN

Type: PAPER
Author(s): ZAKERI S.*,ABOUEI MEHRIZI A.,AGHAJARI M.,NOURIZADEH S.,RAEISI AHMAD,DINPARAST JADID N.
 
 *MALARIA RESEARCH GROUP (MRG), BIOTECHNOLOGY DEPARTMENT, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Name of Seminar: NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN
Type of Seminar:  CONGRESS
Sponsor:  IRANIAN BIOTECHNOLOGY ASSOCIATION
Date:  2005Volume 4
 
 
Abstract: 

Analysis of the Plasmodium vivax merozoite surface protein-1 (Pvmsp-1, as malaria vaccine candidate) has served to enhance the findings of epidemiologic and molecular biological surveys on the origins of the Iranian strains of P. vivax in the north. Therefore, this study was undertaken to provide data on the distribution of the genetic diversity of P. vivax populations in north by using sequences analysis of variable block 5 of the Pvmsp-1 gene and also to define the origin of the resurgent parasite in north with comparing parasite from highly endemic areas in south and also with other parasite populations from different malaria endemic areas of the world. 52 northern and 94 southern isolates were sequenced and Type 1, Type 2 and Type 3 were present in both study areas. However, the most prevalent type in north was Type 1 and among southern isolates were Type 2 sequences. The high prevalence of Type 3 in southern isolates (26.5%) suggested that recombination is the main mechanism in generation of new Pvmsp-1 allele in this area. We have also found 7 and 24 different alleles among 52 northern and 94 southern sequenced samples, two of which were newly identified in this study. Based on nucleotide sequences of Pvmsp-1 gene phylogenetic tree was constructed for north and southern samples, along with isolates from Azerbaijan, Indian, South and North Korea and Thailand. The results showed that genetic relationship of 100% similarity among northern and southern isolates and also 100% similarity with Bangladeshi, Indian and Azerbaijani isolates.
In conclusion, the present study determined that southern isolates are more polymorphic compare with northern isolates and this may suggest that the parasite in re-emerged malaria area is more isolated than in areas in south with high displacement of populations. This information could also be necessary for malaria vaccine development to improve control measures.

 
Keyword(s): MALARIA, PLASMODIUM VIVAX, MEROZOITE SURFACE PROTEIN, GENETIC DIVERSITY, POLYMORPHISM, IRAN
 
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