Paper Information

Title: 

DETECTION OF POLYMERASE CHAIN REACTION-AMPLIFIED IMMUNODEFICIENCY VIRUS PROVIRAL DNA WITH A DIGOXIGENIN-LABELED NUCLEOTIDE AND ENZYME-LINKED OLIGOSORBENT ASSAY

Type: PAPER
Author(s): BAGHERI REZVAN*,SARAMI FOROUSHANI R.,KHANAHMAD H.,SABAHI F.,ABACHI M.,ESMAEILI REZVAN,ADELI AHMAD,AMINI BAVIL OULIAEI S.,MAHBOUDI FEREYDOUN*
 
 *BIOTECHNOLOGY RESEARCH CENTER, PASTEUR INSTITUTE, TEHRAN, IRAN
 
Name of Seminar: NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN
Type of Seminar:  CONGRESS
Sponsor:  IRANIAN BIOTECHNOLOGY ASSOCIATION
Date:  2005Volume 4
 
 
Abstract: 

An enzyme-linked oligosorbent assay (ELOSA) was developed for the detection on microtiter plates of PCR amplified human immunodeficiency DNA. In this nonisotopic PCR assay designated PCR-ELOSA, fragment of the HIV gag gene from peripheral blood mononuclear cells (PBMCs) was first amplified with a digoxigenin-labeled amplified DNA segment was reacted in solution with a probe labeled with biotin. Hybrids were captured in microtiter plate coated with streptavidin. Specific bound hybrids were detected by the addition of on enzyme-labeled antibody against digoxigenin and chromogenic substrate. This method was developed to increase both sensitivity of detection of PCR product.

 
Keyword(s): HIV, NESTED PCR, PCR- EL
 
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