Paper Information

Title:  A MODIFIED ACID PRECIPITATION PROCEDURE FOR PURIFICATION AND REFOLDING OF RECOMBINANT HUMAN PROINSULIN
Type: PAPER
Author(s): ALI AHMADI ATOUSA*,MORADI ALI,OMIDI MARYAM,SARDARI SOROUSH,TAHERIEH A.,JEBELI M.R.,SADEGHCHEH T.,ADELI AHMAD,MAHBOUDI FEREYDOUN*
 
 *BIOTECHNOLOGY DEPT., PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Name of Seminar: NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN
Type of Seminar:  CONGRESS
Sponsor:  IRANIAN BIOTECHNOLOGY ASSOCIATION
Date:  2005Volume 4
 
 
Abstract: 

In this study a rapid, single-step purification method for recombinant human proinsulin, expressed in E. coli cells is reported and the subsequent refolding procedure and characterization of the refolded product are described. The inclusion bodies (IBs) of protein A fusion human proinsulin were solubilized and converted to hexasulfonate derivatives. Then the fusion protein was purified using a modified acid precipitation protocol up to 80% purity. Purified fusion protein was refolded by two methods and the efficiency of these methods was evaluated using HPLC and native PAGE. The best reaction time for refolding step was determined to be about 16 hours.

 
Keyword(s): RECOMBINANT PROINSULIN, PURIFICATION, REFOLDING, E. COLI
 
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