Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Title: 

GENETIC DIVERSITY IN ALFALFA (MEDICAGO SATIVA L.) POPULATIONS AS REVEALED BY RETROTRANSPOSON BASED MARKERS

Type: PAPER
Author(s): AZIZI H.*,ABDOLLAHI MANDOULAKANI B.
 
 *DEPARTMENT OF AGRONOMY AND PLANT BREEDING, FACULTY OF AGRICULTURE, URMIA UNIVERSITY, URMIA, IRAN
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

RETRO TRANSPOSONS ARE COMMON COMPONENTS OF PLANT GENOMES. THEIR ABUNDANCE, DISPERSION, UBIQUITY AND ABILITY TO TRANSPOSE MAKE THEM GOOD POTENTIAL MARKERS. IN THE CURRENT STUDY, MULTILOCUS PCR-BASED TECHNIQUES, IRAP AND REMAP WERE USED TO DETECT INTEGRATION EVENTS OF RETRO TRANSPOSON FAMILIES TMS1RET1, LORE1, LORE2, TPS12A AND TPS19 IN MEDICAGO SATIVA GENOME. IRAP AND REMAP MARKERS, DERIVED FROM THESE RETRO ELEMENTS, WERE ALSO USED TO STUDY GENETIC DIVERSITY AMONG AND WITHIN 80 TETRAPLOID ALFALFA GENOTYPES FROM EIGHT POPULATIONS. RESULTS INDICATED THAT RETRO TRANSPOSONS TMS1RET1, LORE1, LORE2 ARE PRESENT AND TRANSPOSOINIONALLY ACTIVE IN M. SATIVA GENOME. A TOTAL OF 101 AND 119 LOCI WERE AMPLIFIED USING 10 AND 14 IRAP AND REMAP PRIMERS, RESPECTIVELY. THE NUMBER OF POLYMORPHIC LOCI WAS 66 AND 62 FOR IRAP AND REMAP, RESPECTIVELY. POPULATIONS WERE GROUPED INTO THREE MAIN CLUSTERS BASED ON 128 IRAP AND REMAP POLYMORPHIC LOCI. THE GENETIC DISTANCE BETWEEN POPULATIONS RANGED FROM 0.08 (GHATAYONJEH-MALEKKANDI AND TURKEY-SAKUEL) TO 0.134 (AZARBAYEJAN-ORDUBAR AND TURKEY-SAKUEL) WITH A MEAN VALUE OF 0.102. AMOVA REVEALED THE HIGHER LEVEL OF GENETIC VARIATION WITHIN POPULATIONS (92%) COMPARED TO AMONG POPULATIONS (8%), INDICATED NO CLEAR DIFFERENTIATION BASED ON THE STUDIED POPULATIONS. MEAN OF HETROZYGOSITY OF POPULATION VARIED FROM 0.238 (TURKEY-SAKUEL) TO 0.257 (MAHALIE-ESFAHANI AND TURKEY1), AVERAGING 0.252. IRAP AND REMAP-BASED CLUSTER ANALYSIS USING COMPLETE LINKAGE ALGORITHM BASED ON DICE SIMILARITY COEFFICIENT IDENTIFIED FIVE HETEROTIC GROUPS IN 80 ALFALFA GENOTYPES THAT COULD BE APPLIED AS PARENTS IN M. SATIVA ALFALFA BREEDING PROGRAMS.

 
Keyword(s): TETRA PLOID ALFALFA (MEDICAGO SATIVA), AMOVA, TMS1RET1 RETROTRANSPOSON
 
 
Yearly Visit 72   tarjomyar
 
Latest on Blog
Enter SID Blog