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Paper Information

Title: 

TRANSFORMING OF B LYMPHOCYTES BY EPSTEIN BARR VIRUS FOR IRANIAN ETHNIC GROUPS

Type: PAPER
Author(s): MOHAMMADI SH.,ASADI M.,IZADPANAH M.,NIKFARJAM L.,SANATI M.H.
 
 
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

IT IS WELL ACCEPTED THAT NORMAL SOMATIC CELLS DO NOT DIVIDE INDEFINITELY IN VITRO. THERE IS ESSENTIAL NEED FOR INFINITE SOURCE OF HUMAN CELLS AND BIOLOGICAL MOLECULES FOR RESEARCHER ALL OVER THE COUNTRY. IRANIAN BIOLOGICAL RESOURCE CENTER (IBRC) HAVE ESTABLISHED IMMORATALIZATION OF B CELLS OF IRANIAN ETHNIC GROUPS. THESE INDIVIDUALS HEVE BEEN INTERVIEWED FOR THEIR ETHNIC ORIGIN TO BE THE SAME FOR 3 GENERATIONS. SO THESE SAMPLE ARE PURE IRANIAN ETHNIC INDIVIDUALS PARTICIPATING IN PRODUCTION OF LYMPHOBLASTOID CELLS FROM PERIFERAL BLOOD.
FICOL HISTOPACK 1077 WAS USED FOR ISOLATION OF PBMC. THE EBV-PRODUCING MARMOSET B-CELL LINE (B95-8) WAS USED FOR SOURCE OF EBV. INTRODUCING EBV TO PBMC TRANSFORMED LYMPHOBLASTOID CELLS COULD BE OBSERVED WITHIN 4-8 WEEKS BY CLUMP FORMATION IN B CELLS.
BESIDES, FIVE VIRUSES DETECTING, CARRIED OUT FOR ALL SAMPLES. 16 STR MARKERS DETERMINED FOR EACH INDIVIDUAL BEFORE AND AFTER IMMORTALIZATION PROCESS TO VERIFY THE ORIGINAL SAMPLE AND PREPARE SAMPLES PROFILE. SO FAR 16 SAMPLES HAVE BEEN TRANSFORMED AND IMMORTALIZED SUCCESSFULLY.

 
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