Paper Information

Title: 

CORYLUS AVELLANA CELL CULTURE AS A SUPPLEMENTARY COMMERCIAL SUPPLY OF PACLITAXEL FOR RARE TAXUS SPP.

Type: PAPER
Author(s): REZAEI AYATOLLAH*,GHANATI FAEZEH
 
 *FACULTY OF AGRICULTURAL SCIENCES, SHAHED UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

PACLITAXEL IS AN EFFECTIVE ANTINEOPLASTIC AGENT, WHICH IS MAINLY PRODUCED BY DIFFERENT SPECIES OF TAXUS. A MAJOR LIMITATION TO THE THERAPEUTIC USE OF PACLITAXEL IS THE SUPPLY OF THE COMPOUND FROM ITS NATURAL SOURCE, THE BARK AND NEEDLES OF LOW-GROWTH TAXUS SPECIES. RECENTLY, A FEW STUDIES HAVE INTRODUCED HAZELNUT (CORYLUS AVELLANA L.) AS SOURCE OF PACLITAXEL- (AND RELATED TAXANES). IN ORDER TO ACHIEVE PRODUCTION OF PACLITAXEL IN CELL CULTURES OF HAZELNUT AND COMPARE IT WITH THE TAXUS, THE INDUCTION OF CALLUS FORMATION FROM SEEDS OF HAZEL USING MS MEDIUM SUPPLEMENTED BY 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) AND BENZYLADENINE (BA) AT DIFFERENT CONCENTRATIONS AND RATIOS WAS STUDIED. SUSPENSION CELL CULTURES WERE ALSO ESTABLISHED IN MS MEDIUM ADDED WITH 2, 4-D (1 MG/L) AND BA (0.5 MG/L). CALLUS INDUCTION AND CELL GROWTH RATE WERE MEASURED AND GROWTH CURVE WAS ALSO DETERMINED. CELL-ASSOCIATED AND EXTRACELLULAR PACLITAXEL WERE QUANTIFIED IN SUSPENSION AT 10, 12 AND 14 DAYS AFTER SUBCULTURE WAS DETERMINED BY HPLC SYSTEM. THE RESULTS SHOWED THAT ON THE GROWTH CURVE BASIS, A COUPLE OF WEEK WAS DESIRED FOR SUBCULTURING IN SOLID AND LIQUID MEDIA. OVER A PERIOD OF ABOUT 6 MONTHS, THE GROWTH RATE IN SOLID AND LIQUID MEDIA WAS ABOUT 5 AND 15 G/WEEK RESPECTIVELY. THE CONTENT OF CELL-ASSOCIATED PACLITAXEL FOR DAYS 10, 12 AND 14 AFTER INOCULATION WAS 1.67, 2.96 AND 4.25 (MG/G DRY WEIGHT) RESPECTIVELY. THE EXTRACELLULAR PACLITAXEL CONTENT FOR ABOVE-MENTIONED DAYS WAS 12.52, 16.16 AND 22.25 (MG/L) RESPECTIVELY. IF WE COMPARE THESE RESULTS WITH THOSE MADE USING TAXUS SHOWS THAT HAZELNUT IN ADDITION TO WIDELY AVAILABLE AND GROWS AT A MUCH FASTER RATE THAN TAXUS, ITS CELL CULTURES ALSO SHOW A GREATER EASE OF GROWTH AND A SIMILAR PRODUCTION IN LESS TIME, SO THAT OPTIMIZATION OF THE CULTURE MEDIUM MAY PROVIDE A GOOD SYSTEM FOR THIS NEW NATURAL SOURCE OF PACLITAXEL.

 
Keyword(s): HAZELNUT (CORYLUS AVELLANA L.), TAXUS, CELL CULTURE, PACLITAXEL
 
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