Paper Information

Title: 

SCREENING OF POLA GENE AMONG IRANIAN THERMOPHILIC BACILLUS SPP.

Type: PAPER
Author(s): MAHMOODNIA S.*,GHARAVI S.,ASGARANI E.
 
 *BIOLOGY DEPARTMENT, FACULTY OF BASIC SCIENCES, ALZAHRA UNIVERSITY, TEHRAN, IRAN
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

DNA POLYMERASES ARE A FAMILY OF ENZYMES RESPONSIBLE FOR REPLICATION, MAINTENANCE AND TRANSMISSION OF GENETIC INFORMATION IN ALL ORGANISMS. BOTH PROKARYOTES AND EUCARYOTES HAVE SEVERAL DNA POLYMERASES; A NUMBER OF THEM ARE INVOLVED IN DNA REPLICATION BUT OTHERS ARE RESPONSIBLE FOR REPAIR AND OTHER ACCESSORY PROCESSES. DNA POLYMERASE HAVE BEEN USED EXTENSIVELY IN MOLECULAR BIOLOGICAL, SPECIALLY IN TWO IMPORTANT TECHNOLOGIES IN MOLECULAR BIOLOGY: PCR AND DIDEOXY DNA CYCLE SEQUENCING. SINCE THE DISCOVERY OF THE FIRST THERMOSTABLE DNA POLYMERASE –TAQ POLYMERASE - AND ITS APPLICATION IN PCR, MOLECULAR BIOLOGY RESEARCH HAS BEEN CONSIDERABLY EXPEDITED AND SEARCH HAS BEEN DIRECTED FOR OTHER THERMOPHILIC DNA POLYMERASES WITH MORE REFINED QUALITIES. THE AIM OF THE PRESENT STUDY WAS TO ASSESS THE POTENTIAL BIOTECHNOLOGICAL CAPABILITIES OF IRANIAN THERMOSTABLE DNA POLYMERASES FROM VARIOUS THERMOPHILIC EUBACTERIA ISOLATED FROM A HOT SPRING IN ARDABIL, IRAN. BIOCHEMICAL AND MICROSCOPIC ANALYSIS SHOWED THAT THEY BELONG TO THE GENUS BACILLUS AND GEOBACILLUS. APPROPRIATE PRIMERS FROM HIGHLY CONSERVED DOMAINS OF THIS GENE IN THERMOPHILIC BACILLUS WERE DESIGNED. FROM THE NINE ISOLATES EXAMINED, FIVE WERE SELECTED FOR FURTHER CHARACTERIZATION OF THE GENE.

 
Keyword(s): THERMOPHILE, THERMOSTABLE DNA POLYMERASE, CONSERVED DOMAIN
 
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