Paper Information

Title: 

THE DRAFT GENOME SEQUENCE OF NESERENKONIA SP STRAIN F ISOLATED FROM ARAN-BIDGOL SALT LAKE IN IRAN

Type: PAPER
Author(s): SARIKHAN SAJJAD,AZARBAIJANI REZA*,PARSA YEGANEH LALEH,SHAHZADEH FAZELI ABOLHASAN,AMOOZEGAR MOHAMMAD ALI,HOSSEINI SALEKDEH GHASEM
 
 *DEPARTMENT OF MOLECULAR BIOLOGY, IRANIAN BIOLOGICAL RESOURCE CENTER, TEHRAN, IRAN
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

THE DRAFT GENOME OF THE AEROBIC, GRAM-POSITIVE, CHEMOORGANOTROPH AND HALOPHILIC NESTERENKONIA SP STRAIN F CONSISTS OF 2,812,133 BP CHROMOSOME (AFRW01000000 ACCESSION NO.). THE GENOME PROJECT DATA IS ALSO AVAILABLE AT GENBANK UNDER THE GENOME PROJECT ID 68173.
NESTERENKONIA IS AN AEROBIC, GRAM-POSITIVE, CHEMOORGANOTROPH, CATALASE POSITIVE, MESOPHILIC AND HALOPHILIC GENUS FROM MICROCOCCACEAE FAMILY. NESTERENKONIA SP STRAIN F WAS ISOLATED FROM ARAN-BIDGOL HYPERSALINE LAKE IN IRAN AND CHARACTERIZED TO PRODUCE THE HALOPHILIC AND ORGANIC SOLVENT TOLERANT Α-AMYLASES. DESPITE OF ITS HIGH POTENTIAL FOR PRODUCING THE VARIOUS NATURAL COMPOUNDS AND SPECIALIZED MECHANISMS FOR ADAPTION TO EXTREME CONDITIONS, THERE IS A LITTLE INFORMATION ABOUT THE GENETIC AND GENOME FEATURES OF THE NESTERENKONIA GENUS.
GENOME SEQUENCING OF NESTERENKONIA SP STRAIN F WAS PERFORMED USING 454 GS-FLX TITANIUM. WE OBTAINED A TOTAL 201,320 RANDOM READS, COVERING A TOTAL OF 44,347,672 BP. THE APPROXIMATE COVERAGE OF NESTERENKONIA SP STRAIN F GENOME WAS 16 FOLD. THE SEQUENCE READS WERE ASSEMBLED INTO 138 CONTIGS USING NEWBLER ASSEMBLER SOFTWARE V. 2.3. THEN, THE RAST ANNOTATION PIPELINE WAS USED TO ANNOTATE THE DNA SEQUENCE.
THE G+C CONTENT OF THE DRAFT GENOME WAS 71.5% THAT WAS SIMILAR TO PREVIOUS REPORT BY STACKEBRANDT ET. AL. THE SUM OF 138 LARGE CONTIGS SIZE OF NESTERENKONIA SP STRAIN F IS 2,812,133 BP. THE DRAFT GENOME CONTAINED 2484 GENES WITH 1794 NON-HYPOTHETICAL AND 690 HYPOTHETICAL PROTEIN CODING SEQUENCES AND 50 STRUCTURAL RNAS.
ANALYSIS OF ANNOTATED GENOME SEQUENCE OF NESTERENKONIA SP STRAIN F REVEALED THE PRESENCE OF GENES INVOLVED IN PRODUCTION OF Α-AMYLASES INCLUDING MALTODEXTRIN GLUCOSIDASE AND ALPHA-GLUCOSIDASE.
NESTERENKONIA SP STRAIN F COMPRISES CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS (CRISPR), WHICH GRANTS AN EFFECTIVE MECHANISM AGAINST FOREIGN GENETIC ELEMENTS AS AN ADAPTIVE IMMUNE SYSTEM (4). THE CRISPR-ASSOCIATED HELICASE CAS3, RECB FAMILY EXONUCLEASE CAS4B AND CRISPR-ASSOCIATED PROTEIN CAS1 HAVE BEEN DETECTED USING CRISPRFINDER ONLINE PROGRAM.

 
Keyword(s): NESTERENKONIA SP. STRAIN F, DRAFT GENOME SEQUENCING, ANNOTATION, CRISPR
 
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