Paper Information

Title:  SOMATIC EMBERYOGENESIS AND PLANTLET REGENERATION IN CROCUS SPECIOSUS
Type: PAPER
Author(s): KARAMIAN R.*
 
 *DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCES, BU-ALI SINA UNIVERSITY, HAMADAN, IRAN
 
Name of Seminar: NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN
Type of Seminar:  CONGRESS
Sponsor:  IRANIAN BIOTECHNOLOGY ASSOCIATION
Date:  2005Volume 4
 
 
Abstract: 

Somatic embryogenesis was induced from shoot meristems of Crocus speciosus. The explants were cultivated at 20oC in darkness on Linsmaier and Skoog medium supplemented with 3% (w/v) sucrose and different growth regulator combinations. The best embryogenic rates were obtained in the presence of 4 mg/l kinetin and 1 mg/l 2,4-D, as approximately 30 % of callus formed on this medium produced somatic embryos. All phases of somatic embryogenesis were observed on half strength MS medium with 1 mg/l ABA. Maturated embryos germinated in the presence of 25 mg/l GA3. Conversion of bipolar embryos to plantlets was obtained when they were transferred to half strength Murashige and Skoog medium supplemented with 1 mg/l IAA and 1 mg/l kinetin at 20oC under a 16/8-h (light/dark) photoperiod.

 
Keyword(s): CROCUS SPECIOSUS, PLANT REGENERATION, SOMATIC EMBRYOGENESIS
 
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