Paper Information

Title: 

SCREENING OF CYCLOTIDES AS ANTIMICROBIAL PEPTIDES FROM SEVERAL VIOLACEAE SPECIES

Type: PAPER
Author(s): ROSHAN AKRAM*,ZARRABI MAHBOOBE,ASGARANI EZAT,KANAANI MOHAMMAD REZA
 
 *DEPARTMENT OF BIOLOGY FACULTY OF BASIC SCIENCE ALZARA UNIVERSITY TEHRAN, IRAN
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

CYCLOTIDES ARE PLANT POLYPEPTIDES CHARACTERIZED BY THEIR UNIQUE CYCLIC CYSTEINE KNOT STRUCTURAL MOTIF. IT IS THOUGHT THAT THEY FUNCTION PRIMARILY AS DEFENSE AGENTS IN PLANTS. BUT THEY ALSO HAVE VARIETY OF OTHER BIOLOGICAL PROPERTIES, INCLUDING ANTI-HIV, ANTIMICROBIAL AND CYTOTOXIC EFFECTS. BECAUSE OF THEIR EXCEPTIONAL STABILITY, THEY HAVE ATTRACTED INTEREST AS POTENTIAL PRIMING MATERIALS FOR PROTEIN ENGINEERING AND DRUG DESIGN. IN THE PRESENT STUDY, WE DETERMINED THE CYCLOTIDES CONTENT OF THE VIOLACEAE FAMILY. THE SAMPLES COLLECTED FROM ZANJAN AND TOTAL DNA WAS EXTRACTED BY CTAB BUFFER. FORWARD AND REVERSE PRIMER AGAINST CONSERVE SEQUENCE IN VBC FAMILY OF VIOLACEAE WERE DESIGNED AND USED TO DETERMINE VBC GENE FROM VIOLA IGNOBILIS, VIOLA OCCULTA AND VIOLA ODORATA BY PCR AMPLIFICATION OF THE VBC GENE. RESULTS SHOWED THE PRESENCE OF VBC GENE FOR THE FIRST TIME IN THESE SPECIES. THE SAMPLES WERE COLLECTED FROM NEARLY SAME LOCATION; THEREFORE THEY HAVE SIMILAR BIOLOGICAL PROPERTIES. IT CAN BE SUGGESTED THAT THE ENVIRONMENTAL CONDITIONS STRONGLY AFFECT ON CYCLOTIDE CONTENT OF VIOLACEAE.

 
Keyword(s): CYCLOTIDES, VIOLACEAE, VBC GENE, PCR
 
Yearly Visit 66   tarjomyar
 
Latest on Blog
Enter SID Blog