Paper Information

Title: 

INHERITANCE MODE OF MICROSATELLITE LOCI AND THEIR USE FOR KINSHIP ANALYSIS IN THE POLYPLOID PERSIAN STURGEON (ACIPENSER PERSICUS)

Type: PAPER
Author(s): MOGHIM M.*,POURKAZEMI M.,TAN S.G.,JAVANMARD A.,SIRAJ S.S.,PANANDAM J.M.,POURGHOLAM R.,KOR D.,TAGHAVI M.J.
 
 *GENETIC DEPARTMENT OF THE CASPIAN SEA ECOLOGY RESEARCH CENTER, SARI, IRAN
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

HETEROSIS IS ONE OF THE COMMON METHOD HAS BEEN EMPLOYED AS A TOOL TO IMPROVE AQUACULTURE PRODUCTIVITY REGARDING TO THEIR EXTERNAL FERTILIZATION AND SIMILAR MATING BEHAVIORS. GENERALLY, A HYBRID FISH EXPECTED TO PRODUCE OFFSPRING WITH REMARKABLE GROWTH CHARACTERISTICS AND TOLERANCE TO ENVIRONMENTAL STRESSES. IDENTIFICATION OF SUITABLE MOLECULAR MARKERS FOR DISTINGUISHING THE HYBRID PROGENY FROM THEIR PARENTS PARTICULARLY IN THEIR EARLY STAGES IS VITAL FOR AQUACULTURE BREEDING PROGRAM. IN THE PRESENT STUDY, TWO F1 HYBRID FAMILIES WAS PRODUCED BY CONTROLLED INTER-SPECIFIC HYBRIDIZATION BETWEEN FEMALE AND MALE OF POLYPLOID PERSIAN STURGEON AND SUBSEQUENTLY GENOMIC DNA WAS EXTRACTED USING THE QIAGEN DNEASY TISSUE KIT AND 11 MICROSATELLITE LOCI WAS USED FOR GENOTYPING ANALYSIS OF PARENTS AND THEIR PRODUCED OFFSPRING. ANALYSIS OF INHERITANCE PATTERN DEMONSTRATED CLASSIC MENDELIAN INHERITANCE OF THESE MARKERS IN THE HYBRID OFFSPRING. ALL LOCI SEGREGATED CO-DOMINANTLY, AND CONFIRMED TO MENDELIAN SEGREGATION AND CAN BE USED FOR PARENTAL ANALYSIS AND POPULATION GENETIC STUDIES. THE RESULT OF PRESENT STUDY COULD BE APPLICABLE FOR PARENTAGE ANALYSIS OF THE HYBRID, WHICH WOULD PROVIDE VALUABLE INFORMATION IN FISH BREEDING PROGRAMS AND FACILITATE UNDERSTANDING THE MOLECULAR DYNAMICS IN THE HYBRIDIZATION OF PERSIAN STURGEON FISH SPECIES FROM DIFFERENT FAMILIES. FURTHERMORE, INFORMATION OF THIS STUDY ABOUT HEREDITY PATTERN OF INVESTIGATED LOCI COULD BE IN CONSTRUCTION OF LINKAGE MAP IN THIS SPECIES FOR QTL MAPPING PURPOSE.

 
Keyword(s): HETEROSIS, INTER-SPECIFIC HYBRIDIZATION, MICROSATELLITE LOCI, PERSIAN STURGEON
 
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