Paper Information

Title: 

IDENTIFICATION AND COMPARISON OF THE CHLOROPLAST DNA TRNL-F OF THE POPULUS CASPICA POPULATIONS FROM THE HYRCANIAN FORESTS; NORTH WEST OF IRAN

Type: PAPER
Author(s): BADBAR MARYAM*,HOSSINZADEH COLAGAR ABASALT,MAHMOUDI OTAGHVARI ARMAN
 
 *DEPARTMENT OF BIOLOGY, FACULTY OF BASIC SCIENCES, UNIVERSITY OF MAZANDARAN, BABOLSAR, IRAN
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

THE GENUS POPULUS IS IMPORTANT MEMBER OF SALICACEAE. IN THE WORLD HAVE BEEN IDENTIFIED MORE THAN 20 SPECIES OF SALIX AND 50 SPECIES OF POPULUS. IN ADDITION HAVE BEEN DESCRIBED HUNDREDS OF VARIETIES AND HYBRIDS. SINCE THE TREES ARE AMPLIFIED IN NATURAL METHODS (SEXUAL REPRODUCTION) AND ALSO IN SHOOT CUTTINGS METHOD, BECAUSE OF HYBRIDIZATION BETWEEN THEM, IDENTIFICATION IS VERY DIFFICULT TO IDENTIFY. POPULUS CASPICA IS NATIVE IN HYRCANIAN FORESTS AND THEY USUALLY GROW IN LIGHT-MOISTURE IS REGIONS. THE PURPOSE OF THIS STUDY IS INDENTIFICATION OF CHLOROPLAST GENOME BASED ON THE NUCLEOTIDE SEQUENCE OF THE NON-CODING REGIONS CPDNA (TRNL-F) IN THE P. CASPICA POPULATIONS AND COMPARISON WITH OTHER POPULUS IN THE NCBI GENE BANK. TWO SAMPLES FROM SEVEN SITES OF P. CASPICA, WERE COLLECTED. TOTAL GENOMIC DNA WAS EXTRACTED FROM FROZEN LEAF TISSUES USING OF THE DOYLE AND DOYLE METHOD. POLYMERASE CHAIN REACTION (PCR) PERFORMED BY USING OF PFU DNA POLYMERASE AND OLIGONUCLEOTIDE PRIMERS UEUFF/UEUFR WHICH DESIGNED BY TABERLET ET AL. (1991). THE CHROMATOGRAMS OF THE DNA SEQUENCING RESULTS WERE PROCESSED AND ANALYZED BY THE BLAST SOFTWARE AND WERE SEQUENCED WITH OTHER SEQUENCES OF PUPULUS FINDING IN GENE BANK WITH CLUSTALW VER.2. RESULTS SHOWED THAT P. CASPICA IN ALL POPULATIONS WITH THE SAME SEQUENCE OF 339 NUCLEOTIDE IS DIFFERENT WITH P. NIGRA (281 NUCLEOTIDE), P. TREMULOIDES (224 NUCLEOTIDE), P. NIGRA (228 NUCLEOTIDE) THAT ARE IN GENE BANK. AS IN ALL POPULATIONS SAMPLES P.CASPICA WERE OBSERVED 112 NUCLEOTIDES SUBSTITUTIONS, 78 INSERTIONS AND 27 GAPS THAT WERE DIFFERED FROM OTHER SEQUENCES. BASED OUR FUNDING, RECOMMEND THAT TRNL-F MARKER CAN BE USED AS A MOLECULAR MARKER FOR IDENTIFICATION PROPOS.

 
Keyword(s): POPULUS CASPICA, POPULUS NIGRA, CHOLOROPLAST DNA, TRNL-F, PCR
 
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