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Paper Information

Title: 

MULTIGENE SEQUENCE-BASED POLYPHASIC IDENTIFICATION AS A HIGHLY RESOLVING APPROACH IN FUNGAL PHYLOGENY

Type: PAPER
Author(s): PAPIZADEH MOSLEM*,SOUDI MOHAMMAD REZA
 
 *MYCOLOGY LABORATORY, BANK OF MICROORGANISMS, IRANIAN BIOLOGICAL RESOURCE CENTER (IBRC), ACADEMIC CENTRE FOR EDUCATION, CULTURE AND RESEARCH (ACECR)
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

RECENT PROGRESSIVE ADVANCES IN FUNGAL PHYLOGENY HAVE INTRODUCED MANY APPROACHES THAT CAN CHALLENGE THE CONVENTIONAL WAY OF CLASSIFICATION. THE ANALYSIS OF CONSERVED GENES, TOTAL AND PARTIAL SECONDARY METABOLITES ANALYSIS AND CELL WALL CHEMOTAXONOMY ARE AMONG THE VERY WELL-KNOWN NOVEL APPROACHES THAT HAVE BEEN USED FOR DEEP CLASSIFICATION OF VARIOUS TAXA. IN THIS STUDY SMALL SUBUNIT (18S) RIBOSOMAL RNA GENE (SSU) AND INTERNAL TRANSCRIBED SPACER (ITS) SEQUENCING WAS USED FOR IDENTIFICATION OF 19 ASCOMYCETES BELONGING TO THE VARIOUS ORDERS OF EUROTIOMYCETES AND DOTHIDEOMYCETES. THE IDENTIFIED SPECIES BELONG TO PENICILLIUM PURPUROGENUM, PENICILLIUM OXALICUM, PENICILLIUM CHRYSOGENUM, PENICILLIUM ROQUEFORTI, PENICILLIUM CRUSTOSUM, ASPERGILLUS TERREUS, ASPERGILLUS SCLEROTIORUM, EMERICELLOPSIS PUSILA, PHOMA LEVEILLEI AND CHAETOMIUM GLOBOSUM SPECIES. GENE BANK NUCLEOTIDE DATABASE WAS USED FOR ALIGNMENT AND TREE DESIGNING USING GENEIOUS AND MEGA5PACKAGES. IT WAS SHOWN THAT THE ALIGNMENT OF SSU SEQUENCES IS NOT HIGHLY RESOLVING BECAUSE IT IS HIGHLY CONSERVED IN DIKARYA AND THE SSU FRAGMENT CAN BE RECOMMENDED ONLY FOR RESOLVING HIGHER TAXA (ORDER TO GENUS). BUT THE ITS SEQUENCING IS A VERY POWERFUL RESOLVING AND STATISTICALLY PROVED APPROACH THAT IN SOME CASES CAN BE USED FOR SUBSPECIES DETERMINATION (GENUS TO SUBSPECIES). TO DESIGN A MULTIGENE PHYLOGENY APPROACH BY SEQUENCE-BASED POLYPHASEC IDENTIFICATION, AFTOL AND GENE BANK DATABASES WERE STUDIED FOR FINDING NEW CONSERVED FRAGMENTS AND IT WAS DECIPHERED THAT TOGETHER WITH ITS FRAGMENT, THE PROTEIN CODING EF1 AND RPB2 FRAGMENTS AND SPECIALLY RPB1 GENE AS THE BEST CANDIDATES CAN BE USED FOR SPECIES DETERMINATION AND DEEP TAXA IDENTIFICATION THAT CAN RESULT IN NOVEL DATA OF EVOLUTIONARY AND PHYLOGENIC RELATIONSHIPS WITH A CONSIDERABLE RESOLVING POWER.

 
Keyword(s): MULTIGENE PHYLOGENY, SSU, ITS, AFTOL, ASCOMYCETOUS YEASTS
 
 
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