Paper Information

Title: 

GENETIC VARIABILITY AND GEOGRAPHIC DIFFERENTIATION AND ENDANGERED MEDICINAL PLANT THYMUS DAENENSIS SUBSP. DAENENSIS REVEALED BY RAPD MARKERS

Type: PAPER
Author(s): ZOWGHI MARYAM,MIRLOHI AGHAFAKHR,RAHIMMALEK MEHDI,TALEBI BADAF MAJID,BAHREININEJAD BABAK
 
 
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

THYMUS DAENENSIS IS A MEDICINAL, AROMATIC PLANT ENDEMIC TO IRAN. AMONG THESE SPECIES T.DAENENSIS IS WIDELY DISTRIBUTED IN WESTERN IRAN AND HAS EXTENSIVE PHARMACEUTICALS APPLICATION. THIS STUDY WAS AIMED TO EVALUATE GENETIC DIVERSITY AND DIFFERENTIATION AMONG POPULATION OF T.DAENENSIS FROM DIFFERENT GEOGRAPHICAL REGIONS OF IRAN USING RAPD MARKERS AND TO DETERMINE WHETHER THE GENETIC DISTANCES AMONG THE ACCESSIONS ARE CORRELATED WITH THEIR GEOGRAPHIC DISTRIBUTION PATTERNS. SIXTEEN PRIMERS WERE USED TO AMPLIFY 145 BANDS OUT OF WHICH 127 (87%) WERE POLYMORPHIC. CLUSTER AND PRINCIPLE COORDINATE ANALYSIS (PCA) SHOWED THAT MOST GENOTYPES WERE GROUPED BASED ON THEIR GEOGRAPHICAL DISTRIBUTION. PCA WAS PERFORMED TO VISUALIZE THE ASSOCIATION AMONG ACCESSIONS IN MORE DETAIL. THE RESULTS SHOW THAT THE FIRST THESE PRINCIPAL COORDINATE EXPLAIN 22.86% OF THE TOTAL VARIATION, AND THEY CORRESPOND LARGELY TO THE RESULTS OBTAINED THROUGH CLUSTER ANALYSIS. ANALYSIS OF MOLECULAR VARIANCE (AMOVA) REVEALED THAT THE DIFFERENCES AMONG POPULATIONS ONLY ACCOUNTED FOR 18.12% OF THE TOTAL VARIATION, WHEREAS DIFFERENCES WITHIN POPULATIONS WERE 81.87%. GENETIC DISTANCES AMONG 4 IRANIAN POPULATIONS SHOWED THAT THE MINIMUM GENE DIVERSITY OVER LOCI WAS OBSERVED IN CHARMAHAL POPULATION (0.2062), WHILE THE HIGHEST GENE DIVERSITY WAS FOUND IN ISFAHAN POPULATION (0.35).THE INFORMATION OBTAINED HERE COULD BE VALUABLE FOR DEVISING STRATEGIES FOR CONSERVATION OF THIS MEDICINAL PLANT.

 
Keyword(s): THYMUS DAENENSIS, MEDICINAL PLANT, RAPD MARKER, GENETIC DIVERSITY
 
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