Paper Information

Title: 

GENETIC ANALYSIS ON MICROSATELLITES FOR STOCK CONSERVATION OF PERSIAN STURGEON (ACIPENSER PERSICUS) OF THE CASPIAN SEA

Type: PAPER
Author(s): MOGHIM M.*,POURKAZEMI M.,TAN S.G.,JAVANMARD A.,SIRAJ S.S.,PANANDAM J.M.,LALOEI F.,KOR D.,TAGHAVI M.J.
 
 *GENETIC DEPARTMENT OF THE CASPIAN SEA ECOLOGY RESEARCH CENTER, SARI, IRAN
 
Name of Seminar: CONGRESS OF IRANIAN GENETICS
Type of Seminar:  CONGRESS
Sponsor:  ZAKHAYERE ZHENETICE JAHADE DANESHGAHI
Date:  2014Volume 1
 
 
Abstract: 

PERSIAN STURGEON A COMMERCIALLY IMPORTANT SPECIES INHABITS MOSTLY THE SOUTHERN BASIN OF THE CASPIAN SEA. CURRENTLY THE EFFICIENCY OF NATURAL REPRODUCTION OF THIS FISH HAS GRADUALLY DECLINED DUE TO OVER FISHING, SPAWNING HABITAT LOSS, AND WATER POLLUTION. INFORMATION ON THE GENETIC DIVERSITY OF PERSIAN STURGEON POPULATIONS IS VITAL FOR THEIR BREEDING MANAGEMENT. IN THE LONG TERM, THE MOST IMPORTANT GOAL OF STOCK MANAGEMENT IS TO CONTROL INBREEDING AND DECREASING OF GROWTH TRAITS. THE OBJECTIVE ON THIS STUDY WAS TO EVALUATE THE GENETIC DIVERSITY OF STURGEONS USING 11 SINGLE LOCUS DNA MICROSATELLITE MARKERS. THE 167 PERSIAN STURGEONS WERE RANDOMLY COLLECTED FROM FOUR LOCATIONS IN COASTAL WATERS OF THE CASPIAN SEA NAMELY, RUSSIA (43), TURKMENISTAN (13), IRAN (81) AND SAFIDROD RIVER. PARAMETERS ESTIMATED INCLUDE NUMBER OF ALLELES, ALLELIC FREQUENCIES, PERCENTAGE OF POLYMORPHIC LOCI, OBSERVED AND EXPECTED HETEROZYGOSITY, LINKAGE DISEQUILIBRIUM, CONFORMITY OF ALLELE FREQUENCIES TO THAT EXPECTED UNDER HARDY–WEINBERG EQUILIBRIUM AND ESTIMATES OF POPULATION DIFFERENTIATION INCLUDING F-STATISTICS AND GENE FLOW, GENETIC SIMILARITY AND DISTANCE. THE MEAN NUMBER OF ALLELES YIELDED BY ALL MICROSATELLITES RANGED IN POPULATIONS TURKMENISTAN, RUSSIA, SAFIDROOD AND IRAN WAS 7.22±2.90, 10.1±5.14, 8.2±3.54 AND 9.90±4.72 RESPECTIVELY. THE MEAN SHANON INDEX WAS 1.64±0.48, 1.82±0.57, 1.69± 0.53 AND 1.82±0.5 RESPECTIVELY. AVERAGE OBSERVED HETEROZYGOSITY AT SAMPLED LOCI WAS HIGH, RANGING FROM 0.56±0.20 (TURKMENISTAN COAST) TO 0.67±0.16 (SAFIDROD RIVER).
THE MOST FREQUENT ALLELE (MFA) FOR LOCUS AFUG68B, AFUG63, AFUG56, AFUG112, AFUG19-1, AFUG19-2, AFUG195, AFUG229, AFUG160, AFUG241 AND AOX27 WERE FREQUENT 259, 165, 181, 242, 206 AND 218BP RESPECTIVELY.
GENERALLY, THE RESULTS OF THIS STUDY SHOWED THAT THESE POPULATIONS BELONG TO SAME SPECIES, HOWEVER BASED ON OF THESE 11 INVESTIGATED LOCI THE HIGHEST SIMILARITY WAS BETWEEN POPULATIONS RUSSIAN – IRAN (0.90) AND THE LOWEST SIMILARITY WAS TURKMENISTAN- SAFIEDROD RIVER (0.79).
THESE POLYMORPHIC MARKERS COULD BE FURTHER EVALUATED FOR THEIR USEFULNESS IN MARKER-ASSISTED SELECTION AS THE PCR METHODOLOGY WE DEVELOPED WOULD EXPEDITE SCREENING OF THE LARGE NUMBERS OF ANIMALS REQUIRED FOR SUCH STUDIES.

 
Keyword(s): PERSIAN STURGEON, MICROSATELLITE, CASPIAN SEA, CONSERVATION, INBREEDING, STOCK MANAGEMENT
 
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