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Paper Information

Title: 

RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF TRICHOMONAS VAGINALIS ISOLATES IN TABRIZ-IRAN

Type: PAPER
Author(s): JAMALI RASOUL*,ZAREI KAR B.*,KAZEMI A.A.H.,ASGHARZADEH M.,YOUSEFI SABER,ESTAKHRI R.,MONTAZER SH.
 
 *TABRIZ UNIVERSITY OF MEDICAL SCIENCE -FACULTY OF MEDICINE, PARASITOLOY DEPARTMENT
 
Name of Seminar: NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN
Type of Seminar:  CONGRESS
Sponsor:  IRANIAN BIOTECHNOLOGY ASSOCIATION
Date:  2005Volume 4
 
 
Abstract: 

Trichomonas vaginalis is the causative agent of human trichomoniasis. It is the most common nonviral sexually transmitted disease. The infection may be asymptomatic or may cause severe vaginitis and cervicitis in women. Despite its high prevalence little is known about its genetic variability and factors’ leading to asymptomatic infections. The random amplified polymorphic DNA (RAPD) technique is a simple method to detect DNA polymorphism. We performed RAPD method with 4 different Random primers (OPD1, OPD2, OPD3, and OPD5) for typing the 120 isolates of Trichomonas vaginalis in Tabriz. Phylogenetic analysis was performed using SPSS program and dendrogram with two distinct clusters was constructed. The asymptomatic isolates tended to form a cluster, separate from symptomatic isolates. Further studies for better understanding the relationship is suggested.

 
Keyword(s): TRICHOMONAS VAGINALIS, RAPD, POLYMORPHISM
 
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