Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Title: 

GENOTYPING OF THE TWO COMMON VARIANTS OF K-CASEIN GENE (CSN3) IN THREE RUSSIAN CATTLE BREEDS, USING PCR-RFLP

Type: SPEECH
Author(s): AHANI AZARI M.*,ROSTAMZADEH J.,GALINA EFIMOVNA S.
 
 *VAVILOV INSTITUTE OF GENERAL GENETICS OF RUSSIAN ACADEMY OF SCIENCES, LABORATORY OF COMPARATIVE ANIMAL GENETICS, RUSSIA, MOSCOW, GSP1 119991, GUBKIN STREET 3
 
Name of Seminar: NATIONAL CONGRESS OF BIOTECHNOLOGY OF IRAN
Type of Seminar:  CONGRESS
Sponsor:  IRANIAN BIOTECHNOLOGY ASSOCIATION
Date:  2005Volume 4
 
 
Abstract: 

The genotypes and allele frequencies for k-casein variants A and B in three herds of Bestoozhevski, Kalmitski and Russian black & white breeds were determined .In this study the size of the screened animals , belonging to studied breeds, were 63, 91 and 32 respectively. Genomic DNA isolated from blood of animals. The polymerase chain reaction was used to amplify a 228-bp fragment from the K-casein gene. Using PCR-RFLP with two primers SGO and SGE and two restriction endonucleases (Hinf I and TaqI), the Distribution of Allele A in these breeds were 0.71, 0.72 and 0.68 and allele B 0.29, 0.28 and 0.32 respectively .The two Alleles A (Thr136, Asp148) and B (Ile136, Ala148) have a great importance in production traits, such as milk yield, protein, fat contents and especially cheesemaking properties. Because of the important role of the B variant on increasing in milk protein and fat content, as well as cheese production, its effect is known to be economically important, and it is considered to be included in dairy breeding programs.

 
Keyword(s): KAPPA-CASEIN, HINF I ,TAQ I, PCR-RFLP
 
Yearly Visit 57   pdf-file tarjomyar
 
Latest on Blog
Enter SID Blog