Paper Information

Title: 

AN IN VITRO METHOD FOR ASSESSING THE EFFICACY OF ANTIVENOM AGAINST H. LEPTURUS

Type: SPEECH
Author(s): PIPELZADEH M.H.*
 
 *DEPARTMENT OF PHARMACOLOGY, MEDICAL SCHOOL, AHWAZ UNIVERSITY OF MEDICAL SCIENCES, AHWAZ, IRAN
 
Name of Seminar: IRANIAN CONGRESS OF TOXICOLOGY
Type of Seminar:  CONGRESS
Sponsor:  SOCIETY OF TOXICOLOGY
Date:  2004Volume 8
 
 
Abstract: 

Using red blood cell fragility test, the anti-haemolytic effectiveness of antivenom serum against Hemiscopius lepturus venom was investigated. Degree of haemolysis produced was measured using ELIZA reading method. Addition of venom (2 to 40µg/ml) to 0.5 ml of 5 % red blood cell suspension produced concentration-dependent haemolysis. Premixing of various concentrations of antivenom (10 to 200µg/ml) with standard concentration of venom (10µg/ml), as well as with red blood cells followed by standard concentration of the venom produced concentration-dependent protection against haemolysis Both methods produced protection against haemolysis of the red blood cells for up to 12 hours at maximum concentration of 200µl/ml. The results suggest that the venom from this scorpion has a prolonged duration of action. Furthermore, although the antivenom against H. lepturus is effective in inhibiting the haemolysis produced by the venom, the duration of protection is relatively short and appropriate measures need to be taken, depending on the patients’ clinical progress,  to re-administer the antivenom at intervals less than 1 2 hours. This suggestion deserves further clinical assessment.

 
Keyword(s): HEMISCORPIUS LEPTURUS; FRAGILITY TEST, HAEMOLYSIS, ANTIVENOM
 
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