Paper Information

Title: 

CONSIDERATION OF INHIBITORY EFFECT OF ETHYLPARATHION ON CHOLINE OXIDASE, TO EVALUATE ITS FEASIBILITY FOR DESIGNING A MONO ENZYMATIC BIOSENSORS

Type: PAPER
Author(s): TAVAKOLI HASSAN,GHOURCHIAN HEDAYAYATOLLAH
 
 
 
Name of Seminar: IRANIAN CONFERENCE ON BIOMEDICAL ENGINEERING
Type of Seminar:  CONFERENCE
Sponsor:  ANJOMANE MOHANDESI PEZESHKI IRAN
Date:  2004Volume 11
 
 
Abstract: 

ORGANOPHOSPHATE PESTICIDES ARE WIDELY USED IN MODERN AGRICULTURE. WORLDWIDE INTOXICATION ATTRIBUTED TO PESTICIDES HAS BEEN ESTIMATED TO BE AS HIGH AS 3 MILLION CASES OF ACUTE, SEVERE POISONING A YEAR, A WIDE VARIETY OF BIOSENSOR TECHNIQUES THAT COULD BE APPLIED TO ENVIRONMENTAL ORGANOPHOSPHATES MEASUREMENT HAVE BEEN REPORTED. IN ELECTROCHEMICAL DETECTION METHOD THAT USES ACETYLCHOLINE CHLORIDE AS SUBSTRATE FOR THE ENZYME MODIFIED ELECTRODE, THREE ENZYMES ARE CO-IMMOBILIZED. THE FIRST, ACETYL CHOLINESTERASE (ACHE), IS SENSITIVE FOR INHIBITOR. THE SECOND ENZYME, CHOLINE OXIDE (CHOX), OXIDIZES THE CHOLINE GENERATED BY THE FIRST ENZYME TO PRODUCE HYDROGEN PEROXIDE, WHICH OXIDIZED AT THE WORKING ELECTRODE BY THE THIRD ENZYME (HORSE RADISH PEROXIDASE). AS A RESULT, THE CURRENT GENERATED IS INVERSELY PROPORTIONAL TO THE AMOUNT OF INHIBITORS PRESENT IN THE SOLUTION. THE CO-IMMOBILIZATION OF THREE ENZYMES, IN DESCRIBED PROCEDURE WAS NOT SUCCESSFUL BECAUSE OF THE CONTINUOUS LEAKING OF CHOX WHEREAS ACHE WAS RETAINED IN APPRECIABLE AMOUNT.

 
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