Paper Information

Title: 

THE EFFECT OC CUCLOPHOSPHAMIDE ON RAT INTESTINE, LIVER AND KIDNEY

Type: SPEECH
Author(s): AHMADIZADEH M.,POURSHLEHI H.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF TOXICOLOGY
Type of Seminar:  CONGRESS
Sponsor:  SOCIETY OF TOXICOLOGY
Date:  2004Volume 8
 
 
Abstract: 

Cyclophosphanide (CYC) ia an inhibitor of DNA syntheses which is widely used for treatment of malignant tumors.
Although this drug causes adverse effects on gastrointestinal and urinary system, but the effect of this agent on these tissues has not been investigated completely, the purpose of this study was to determine the effect of CYC on rat liver, small intestine and kidney epithelial cells. Animals were given CYC (dissolved in normal saline) orally at doses of 70,280 and 560mg/kg, and control group received vehicle only. The rats were treated for 4 consecutive days.24 hr after last treatments all animals were killed with sodium thiopental and blood was collected for determination of SGPT, SGOT, AIP BUN and creatinine. The liver, small intestine and kidney tissues were removed, fixed in formalin 10% and processes for light microscopy using H&E, AB and PAS staining methods. Biochemically CYC caused dose dependent elevation of SGPT, SGOT, ALP, BUN and creatinine, Histopatholgically, this drug induced dose dependent cell injury I the liver (hepatocytes). Small intestine (enterocytes) and kindney (proximal convoluted tubulat cells) Cytochemically, CYC caused dose dependent alterations of glycoprotein cells. The results of the present study suggested that the liver, small intestine and kindney tissues of rat may have capacity to biotransforme CYC into toxic metabolites.

 
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