Paper Information

Title:  HUMAN PAPILLOMA VIRUS AND TP53 GENE MUTATION IN ORAL TONGUE SCC AND THEIR CORRELATION WITH TUMOR CHARACTERISTICS; A MULTI-CENTER STUDY
Type: PAPER
Author(s): MAHDIZADEH J.*
 
 *
 
Name of Seminar: INTERNATIONAL CONNGRESS OF IRANIAN SOCIETY OF OTOLARYNGYOLOGY / HEAD AND NECK SURGERY
Type of Seminar:  CONGRESS
Sponsor: 
Date:  2010Volume 1
 
 
Abstract: 

INTRODUCTION: HEAD AND NECK SQUAMOUS CELL CARCINOMA (HNSCC) IS THE SIXTH MOST COMMON CANCER WORLDWIDE; THE MOST COMMON SITE OF ORAL CANCER DEVELOPMENT IS THE ANTERIOR TWO-THIRDS OR THE ORAL TONGUE. OVER THE LAST FEW YEARS IT HAS BECOME CLEAR THAT HUMAN PAPILLOMA VIRUS (HPV) IS ASSOCIATED WITH A SUBSET OF HNSCC AND IT HAS BEEN RECENTLY SUGGESTED TO BE CONSIDERED A RISK FACTOR BESIDES THE TOBACCO AND HEAVY ALCOHOL CONSUMPTION.
OBJECTIVE: THE PURPOSE OF THIS STUDY WAS TO INVESTIGATE THE PREVALENCE OF HPV INFECTION IN PATIENTS WITH SQUAMOUS CELL CARCINOMA (SCC) OF THE TONGUE AND SUBSEQUENTLY ITS SIGNIFICANCE IN CERVICAL LYMPH NODE METASTASES AND TUMOR DIFFERENTIATION.
MATERIALS: SECTIONS OF A FORMALIN- FIXED, PARAFFIN-EMBEDDED TISSUE BLOCK FROM A HUNDRED AND FIFTEEN HISTOLOGICALLY CONFIRMED TONGUE SCC PATIENTS, HAVING UNDERGONE PRIMARY SURGICAL TREATMENT FROM APRIL 2000 THROUGH MARCH 2007 WERE ENROLLED IN THIS STUDY. PATIENTS’ DEMOGRAPHIC AND CLINICAL CHARACTERISTIC DATA WERE COLLECTED.
METHODS: POLYMERASE CHAIN REACTION (PCR) WAS PERFORMED USING
B-GLOBIN PRIMERS FOR THE CONFIRMATION OF AMPLIFIABLE DNA IN THE TISSUE EXTRACTS. B-GLOBIN-POSITIVE SAMPLES WERE ANALYZED BY POLYMERASE CHAIN REACTION FOR THE DETECTION OF HPV 16 AND 18 INFECTION.
RESULTS: THE FREQUENCIES OF HPV 16 AND HPV 18 INFECTION WERE 10.6% AND 18% RESPECTIVELY. YOUNG PATIENTS (AGED BELOW 45 YEARS) COMPRISED 20.4% OF ALL PATIENTS. THERE WAS NOT A SIGNIFICANT ASSOCIATION BETWEEN HPV 16 OR HPV 18 PRESENCE AND HIGHER STAGES OF THE TUMOR, TUMOR DIFFERENTIATION OR THE PRESENCE OF NODAL METASTASIS.
CONCLUSION: ALTHOUGH THE ASSOCIATION BETWEEN HNSCC AND HPV INFECTION IS BEING RECOGNIZED AND REPORTED, OUR DATA IMPLICATES THAT HPV INFECTION MAY NOT PLAY A SIGNIFICANT ROLE IN ORAL TONGUE SCC PATHOGENESIS, DIFFERENTIATION OR METASTASIS.

 
Keyword(s): HPV, SCC, TONGUE
 
Yearly Visit 3  
 
Latest on Blog
Enter SID Blog