Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Title: 

PREVALENCE OF Y SHRONOSOME MICRODELETIONS IN 40 IRANIAN INFERTILE MEN

Type: PAPER
Author(s): MEHRSAI ABDOLRASOL*,PPURMAND GHOLAMREZA,SINA ALIREZA,MANSOORI DARYOUSH,TAHERIMAHMOUDI MOHSEN,HEMMATIAN M.A.
 
 *
 
Name of Seminar: CONGREH ANJOMAN UROLOGY IRAN
Type of Seminar:  CONGRESS
Sponsor:  ANJOMAN OROLOGY
Date:  2005Volume 8
 
 
Abstract: 

INTRODUCTION AND OBJECTIVES: TO DETERMINE THE FREQUENCY OF Y CHROMOSOME AZF (AZOOSPERMIA FACTOR) SUBREGIONS’ MICRODELETIONS IN PATIENTS WITH IDIOPATHIC NONOBSTRUCTIVE AZOOSPERMIA AND SEVERE OLIGOZOOSPERMIA.
METHODS: SUBJECTS INCLUDED 40 MEN WHO HAD BEEN REFRRED TO INFERTILITY CLINICS FOR ASSISTED REPRODUCTION, 37 WERE AZOOSPERMIAC AND 3 HAD SEVERE OLIGOSPERMIA. MEDICAL HISTORY AND PHYSICAL EXAM REVEALED NO EVIDENCE OF INFECTION, OBSTRUCTION OF SEMINAL TRACT, ENDOCRINE FAILURE OR CHROMOSOMAL ANOMALIES. HORMONAL STUDY WAS PERFORMED FOR ALL PATIENTS. 26 MEN HAD TESTCULAR BIOPSIES INCLUDING 11 PATIENTS WITH HYPO SPERMATOGENESIS, 9 PATIENTS WITH MATURATION ARREST, 4 PATIENTS WITH SERTOLI CELL ONLY SYNDROME AND 2 PATIENTS WITH TUBULAR SCLEROSIC. IN 14 MEN WHO DID NOT HAVE TESTICULAR BIOPSY MULTIPLE EPIDIDYMAL AND TESTICULAR SPERM ASPIRATIONS UNDER ANESTHESIA FAILED AND TESTICULAR SPERM EXTRACTION WAS SUBSEQUENTLY PERFORMED FOR ICSI. DNA WAS ISOLATED FROM BLOOD SAMPLES. POLYMERASE CHAIM REACTION (PCR) AMPLIFICATION OF 11 LOCI SPAMMING THE AZF A, AZF B AND AZF C CUBREGIONS OF THE Y CROMOSOME USING, SY81, SY83, SY127, SY130, SY131, SY147, SY149, SY156, SY176, SY254 AND, SY279 WAS PERFORMED.
RESULTS: MICRO DELETIONS OF THE Y CHROMOSOME WERE FOUND IN TWO OF THE PATIENTS (50%), BOTH OF THEM HAD AZOOSPERMIA. DELETIONS WERE RESTRICTED TWO DAZ (DELETED IN AZOOSPERMIA) LOCUS IN AZFCUBREGION. ONE OF PATIENTS HAD A HISTORY OF CRYPTORCHIDISM AND THE SECOND HAD UNDERGONE A LEFT SIDE VARICOCELECTOMY. TESTICULAR PATHOLOGY WAS SERTOLI CELL ONLY SYNDROME IN BOTH OF THEM.
CONCLUSIONS: OUR EXPERIENCE ADDS TO THE CURRENT LOGIC THAT MEN WITH AZOOSPERMIA OR SEVERE OLIGOSPERMIA SHOULD BE EVALUATED FOR ASSISTED REPRODUCTIVE TECHNIQUES.

 
Keyword(s): CHROMOSOME, INFERTILE, AZF
 
 
Yearly Visit 45   tarjomyar
 
Latest on Blog
Enter SID Blog