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Paper Information

Title: 

ER-B EXPRESSION DURING MOUSE PROSTATE CARCINOGENESIS

Type: PAPER
Author(s): HOOSHDARAN M.Z.*,RAGHOW S.,STEINER M.S.
 
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Name of Seminar: CONGREH ANJOMAN UROLOGY IRAN
Type of Seminar:  CONGRESS
Sponsor:  ANJOMAN OROLOGY
Date:  2005Volume 8
 
 
Abstract: 

PROPOSE: INTRODUCTION: PROSTATE CANCER IS THE MOST COMMONLY DIAGNOSED NEOPLASM AND THE SECOND LEADING CAUSE OF DEATH IN THE UNITED STATES. ALTHOUGH THE ROLE OF ESTROGEN IN FEMALE REPRODUCTION HAS BEEN WELL DOCUMENTED, ITS ROLE IN MALE REPRODUCTION IS NOT CLEAR. ESTROGEN HAS BEEN IMPLICATED IN PROSTATE CARCINOGENESIS AND TUMOR PROGRESSION AS REVEALED BY BOTH EPIDEMIOLOGICAL AND EXPERIMENTAL DATA. IT IS GENERALLY ACCEPTED THAT ESTROGEN INFLUENCES PROSTATE GROWTH INDIRECTLY THROUGH THE HYPOTHALAMIC AND PITUITARY HORMONES. WE INVESTIGATED THE EFFECT OF TOREMIFENE ON ESTROGEN RECEPTOR-A (ERA) AND ESTROGEN RECEPTOR-B (ERB) EXPRESSION IN PROSTATE TISSUE OF TRAMP MICE.
METHODS & MATERIALS: CROSSBRED [TRAMP
´FVB] F1 MICE WERE USED FOR THIS STUDY. TWO GROUPS OF 20 ANIMALS EACH RECEIVED EITHER A TOREMIFENE PELLET (10 MG/KG/DAY, 90-DAY SLOW-RELEASE PELLET) OR A PLACEBO PELLET. PROSTATE TISSUES FROM FIVE ANIMALS IN EACH GROUP WERE HARVESTED AT 7, 10, 15, AND 20 WEEKS OF AGE, FORMALIN-FIXED, AND PARAFFIN-EMBEDDED. TISSUE SECTIONS (5 MM THICK) WERE IMMUNOSTAINED FOR ERA AND ERB EXPRESSION RESULTS: ERA WAS PRESENT IN THE CYTOPLASM OF PROSTATE EPITHELIUM OF THE PLACEBO-TREATED MICE, AND, INTERESTINGLY, IN THE CYTOPLASM AND NUCLEUS OF THE TOREMIFENE-TREATED ANIMALS. EVEN THOUGH THE PERCENTAGE OF ERA IN THE NUCLEUS DECLINED WITH AGE, IT SHOWED THAT TOREMIFENE HAS A ROLE IN ACTIVATING OR MAINTAINING ACTIVITY OF ERA. ERB WAS PRESENT IN THE NUCLEUS AND CYTOPLASM OF PLACEBO- AND TOREMIFENE-TREATED MICE UNTIL WEEK 20. AT 20 WEEKS OF AGE, TISSUES FROM ALL PLACEBO-TREATED ANIMALS SHOWED ADVANCED PROSTATIC EPITHELIAL NEOPLASIA (PIN), AND ERB WAS UNDETECTABLE. INTERESTINGLY, AT 20 WEEKS OF AGE, ERB WAS EXPRESSED IN THE TOREMIFENE-TREATED ANIMALS, BUT NO PIN OR TUMORS WERE OBSERVED IN THESE ANIMALS.
DISCUSSION & CONCLUSION: TOREMIFENE HAS A POTENTIAL ROLE IN INHIBITING PIN, AND ERB MAY HAVE AN IMPORTANT ROLE IN PROSTATE CANCINOGENESIS AND PERHAPS IN PROSTATE CANCER THERAPY.

 
Keyword(s): ERA, ERB, TRAMP MICE
 
 
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