Paper Information

Title: 

ANTIOXIDANT ACTIVITY OF HEDERA HELIX LEAVES EXTRACT

Type: POSTER
Author(s): KHAN S.,SHAH A.J.,ALI A.N.A.,GILANI A.H.*
 
 *NATURAL PRODUCT RESEARCH LAB., DEPARTMENT OF BIOLOGICAL AND BIOMEDICAL SCIENCES, AGA KHAN UNIVERSITY MEDICAL COLLEGE, KARACHI, PAKISTAN
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Oxygen-reactive species, in particular free radicals have been recognized to be involved in several diseases, including neurological, cardiovascular and inflammatory disorders. Hedera helix (H. helix), is an evergreen medicinal and ornamental plant. It has been used in folk herbal remedies, especially in the treatment of rheumatism, gout, and bronchitis. Its leaves are reported to have antibacterial, antirheumatic, antispasmodic, astringent, vasoconstrictor, vasodilator and stimulant properties. Considering the medicinal value and the fact that there is no information available to the author’s knowledge regarding the antioxidant potential of H. helix, we have undertaken the present investigation to evaluate the antioxidant property of H. helix leave extract and its possible mechanism.
Methods: The H. helix leave extract was evaluated for its antioxidant property using DPPH free radical scavenging, free radical mediated DNA damage, lipid per oxidation and free radical-induced hemolysis of red blood cells assays. Quercetin, rutin and ascorbic acid were used as reference antioxidants.

Results:
Data obtained from the DPPH free radical scavenging assay showed that H. helix extract (5-200µg/ml) showed concentration-dependent increase in the antioxidant properties (IC50 = 51±2.6µg/ml). Results obtained from the free radical mediated DNA damage, lipid per oxidation and free radical-induced hemolysis of red blood cells assays were in support to those obtained with DPPH assay.
Conclusions: Our experimental evidence suggests that the H. helix extract possesses antioxidant property by inhibiting the free radical mediated DNA damage and lipid peroxidation and this antioxidant activity may explain some of its medicinal uses. Bio assay direc.

 
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