Paper Information

Title: 

STUDY OF ANTI-INFLAMMATORY EFFECT OF ROSE BANGAL IN INFLAMMATORY MACROPHAGES

Type: POSTER
Author(s): ZAMANI TAGHIZADEH RABE SH.,MOUSAVI SH.,MAHMOUDI M.
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Inflammation is a complex process that related to inflammatory disease such as myocard infarction, rheumatoid arthritis, parkinson and multiple sclerosis. Inducible nitric oxide synthase (iNOS), cyclooxygenase II (COX-II) and release of their products (nitric oxide (NO) and prostaglandin E2 (PGE2)) by inflammatory macrophages have critical role in inflammatory conditions. Anti-inflammatory drugs have unpleasant side effect and sometimes are not effective. So, scientists try to find more effective compound. Rose Bengal (RB) is water soluble xanthine dye that has been used in medicine as a safe compound. In this study, anti-inflammatory effect of Rose bangal was investigated in inflammatory macrophages in vitro.
Methods: J774A.1 macrophages (125×103 cell/well in 96-well plate) were treated with bacterial lipopolysachharide (stimulator for iNOS and COX-II and production of pro-inflammatory cytokines) and different concentration of Rose Bengal (10-200 micromolar) and incubated in 37oc. After 18 hours, NO production (by Griess method) and PGE2, TNF-α and IL-1 (By ELISA) were measured. Wester blotting method was used for quantitative measurement of iNOS and COX-II expression.
Results: Rose Bengal caused a significant decrease (p<0.001) in the production of NO, PGE2 and pro-inflammatory cytokines in a dose-dependent manner. This decrease was associated with the decrease in iNOS and COX-II expression. So, these results showed significant anti-inflammatory effect of Rose bangal and the mechanism of its action.
Conclusion: Rose Bengal could be considered as a promising anti-inflammatory agent for the treatment of inflammatory diseases in future.

 
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