Paper Information

Title: 

MEASUREMENT OF TESTOSTERONE IN RIGHT AND LEFT CA1 REGION OF HIPPOCAMPUS IN INTACT AND CASTRATED MALE RATS BEFORE AND AFTER SPATIAL LEARNING

Type: SPEECH
Author(s): MOHADES GISOU,NAGHDI N.,KHAMENEH SAEID
 
 
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Numerous studies have suggested that sex steroids and their receptors exist in hippocampus and affect spatial learning and memory. This study designed to measure testosterone level and to assess the effect of spatial learning on its amount in left and right hippocampus of adult male rats. Sixteen rats were divided to noncastrated and castrated groups, then trained in Morris water maze. Another 40 animals were divided to four groups RCNC, LCNC, RCC and LCC. All of animals were trained in MWM. Microdialysis was performed for four animals in each group and steroid contents of samples were analyzed through HPLC/ UVD method. Our results show no significant differences between control and castrated animals in spatial learning after four days training. Gonadectomy did not change testosterone level in CA1 region of hippocampus. Significant differences are indicated in testosterone level between left and right hippocampus, in favor of left side in all groups. No significant differences were seen in testosterone levels in hippocampus between before and after training, except in right hippocampus of non-castrated group. In conclusion, our findings show that castration does not affect learning. Testosterone, as a neurosteroid, exists in CA1 region of hippocampus and training could decrease its level only in right hippocampus significantly.

 
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