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Paper Information

Title: 

EXISTENCE OF CANNABINOID CB1 AND VANILLOID VR1 RECEPTORS WITHIN CORPUS CAVERNOSUM AND THERAPEUTIC EFFECTS OF THE ENDOCANNABINOID ANANDAMIDE ON NEUROGENIC RELAXATION OF CORPORAL TISSUE FROM DIABETIC RATS

Type: SPEECH
Author(s): GHASEMI M.*,SADEGHIPOUR H.,MANI A.R.,DEHPOUR A.R.
 
 *DEPARTMENT OF PHARMACOLOGY, TEHRAN UNIVERSITY OF MEDICAL SCIENCES (TUMS)
 
Name of Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Type of Seminar:  CONGRESS
Sponsor:  PHYSIOLOGY AND PHARMACOLOGY SOCIETY, MASHHAD UNIVERSITY OF MEDICAL SCIENCE
Date:  2007Volume 18
 
 
Abstract: 

Introduction: Neurogenic relaxation of corpus cavernosum, by nonadrenergic noncholinergic nerves, plays a crucial role in penile erection which could be impaired by pathophysiological conditions such as diabetes. The aim of this study was to verify the existence of cannabinoid and vanilloid receptors within corpus cavernosum and to examine the therapeutic effects of the endocannabinoid anandamide on neurogenic relaxation of this tissue in diabetic rats.
Methods: Corporal tissues of control or diabetic rats (8 weeks after streptozocin administration, 45mg/kg, i.p.) were isolated for 1 performing western blotting of cannabinoid CB1, CB2 and vanilloid VR1 receptor and 2) relaxant responses to electrical field stimulation (EFS) in organ bath. We further evaluated the ability of acute anandamide administration in vitro to improve EFS-induced relaxation of diabetic strips (n=6 per group).
Results: Western blotting revealed the existence of CB1 and VR1 receptors, but not CB2 receptors, within the cavernosal strips. There was a significant impairment (P<0.001) in relaxation to EFS in precontracted (phenylephrine, 7.5µM) diabetic strips compared with controls. Anandamide significantly (P<0.01) potentiated the impaired neurogenic relaxation in diabetic groups dose-dependently (0.3, 1, 3µM) which was antagonized by either of CB1 antagonist AM251 (3µM) and VR1 antagonist capsazepine (10µM) but not by CB2 antagonist AM630 (3µM).
Discussion: For the first time, we demonstrated that cannabinoid CB1 and vanilloid VR1 receptors exist within the rat corpus cavernosum and endocannabinoids such as anandamide via involving these receptors can have improving effects on the neurogenic relaxation of corpus cavernosum from diabetic rats.

 
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